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scHNTL: single-cell RNA-seq data clustering augmented by high-order neighbors and triplet loss.
Bioinformatics
January 2025
School of Computing and Artificial Intelligence, Southwest Jiaotong University, Sichuan 611756, China.
Motivation: The rapid development of single-cell RNA sequencing (scRNA-seq) has significantly advanced biomedical research. Clustering analysis, crucial for scRNA-seq data, faces challenges including data sparsity, high dimensionality, and variable gene expressions. Better low-dimensional embeddings for these complex data should maintain intrinsic information while making similar data close and dissimilar data distant.
View Article and Find Full Text PDF[Colorectal surgery in multimorbid patients].
Chirurgie (Heidelb)
January 2025
Klinik für Allgemein- und Viszeralchirurgie, SRH Zentralklinikum Suhl, Albert-Schweitzer-Straße 2, 98527, Suhl, Deutschland.
Colorectal surgery in multimorbid patients requires a comprehensive interdisciplinary planning of the treatment approach, from preoperative to posthospital care, in order to minimize complications and improve the patient's outcome. Therefore, the integration of the outpatient and inpatient sectors is essential as is a perioperative interdisciplinary coordinated approach. Preoperatively, all possible risks of concomitant diseases must be considered and optimized if necessary.
View Article and Find Full Text PDFPrioritizing questions and topics for the development of guidelines and consensus-based recommendations supported by ILAE: A scoping review and proposal of prioritization criteria.
Epilepsia
January 2025
Department of Clinical Neurosciences, University of Calgary, Calgary, Alberta, Canada.
Clinical practice guidelines (CPGs) and consensus-based recommendations (CBRs) require considerable effort, collaboration, and time-all within the constraints of finite resources. Professional societies, such as the International League Against Epilepsy (ILAE), must prioritize what topics and questions to address. Implementing evidence-based care remains a crucial challenge in clinical practice.
View Article and Find Full Text PDFPhosphate Limitation Enhances Heterologous Enzyme Production in : Mechanistic Insights and Universal Applicability.
J Agric Food Chem
January 2025
State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi 214122, China.
is one of the commonly used hosts for heterologous enzyme expression, depending on media rich in carbon, nitrogen, and phosphate sources for optimal growth and enzyme production. Interestingly, our investigation of maltotetraose-forming amylase, a key enzyme for efficient maltotetraose synthesis, revealed that phosphate limitation significantly enhances the growth rate and production of heterologous enzymes in recombinant . Under phosphate-limited conditions in a 15 L fermenter, the enzyme activity reached 679.
View Article and Find Full Text PDFRadiogenomic explainable AI with neural ordinary differential equation for identifying post-SRS brain metastasis radionecrosis.
Med Phys
January 2025
Deparment of Radiation Oncology, Duke University, Durham, North Carolina, USA.
Background: Stereotactic radiosurgery (SRS) is widely used for managing brain metastases (BMs), but an adverse effect, radionecrosis, complicates post-SRS management. Differentiating radionecrosis from tumor recurrence non-invasively remains a major clinical challenge, as conventional imaging techniques often necessitate surgical biopsy for accurate diagnosis. Machine learning and deep learning models have shown potential in distinguishing radionecrosis from tumor recurrence.
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