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Efficient induction of inner ear hair cell-like cells from mouse ES cells using combination of Math1 transfection and conditioned medium from ST2 stromal cells.
Stem Cell Res
August 2017
Department of Pathogen, Infection and Immunity, Nara Medical University, Kashihara, Nara, Japan. Electronic address:
Article Synopsis
- - The study aimed to improve the induction of inner ear hair cell-like cells (HC-like cells) from embryonic stem cells (ES cells) by combining two existing methods, one utilizing ST2 stromal cell-conditioned medium and the other involving a Math1 gene transfer technique.
- - Using Math1-ES cells and culturing them with doxycycline in the conditioned medium resulted in about 30% of the cells differentiating into HC-like cells, compared to only 20% with each method used separately.
- - The combined method not only increased HC-like cell production but also indicated its potential to induce specific cochlear and vestibular cell types, with continuous Math1 induction favoring cochlear marker development.
Induction of inner ear hair cell-like cells from Math1-transfected mouse ES cells.
Cell Death Dis
July 2013
Department of Pathogen, Infection and Immunity, Nara Medical University, Kashihara, Nara, Japan.
Math1, a basic helix-loop-helix transcription factor homolog of the Drosophila atonal gene, is considered to be a key factor for induction of sensory hair cells (HCs) during development of the organ of Corti or cochlea. Although embryonic stem (ES) cells are able to produce HC-like cells, the role of Math1 in induction of those cells has not been thoroughly elucidated. In the present study, we introduced Math1 into ES cells in order to achieve efficient generation of HC-like cells.
View Article and Find Full Text PDFIn vitro differentiation of mouse embryonic stem cells into inner ear hair cell-like cells using stromal cell conditioned medium.
Cell Death Dis
May 2012
Department of Pathogen, Infection and Immunity, Nara Medical University, Nara, Japan.
Hearing loss is mainly caused by loss of sensory hair cells (HCs) in the organ of Corti or cochlea. Although embryonic stem (ES) cells are a promising source for cell therapy, little is known about the efficient generation of HC-like cells from ES cells. In the present study, we developed a single-medium culture method for growing embryoid bodies (EBs), in which conditioned medium (CM) from cultures of ST2 stromal cells (ST2-CM) was used for 14-day cultures of 4-day EBs.
View Article and Find Full Text PDFWnt signaling induces proliferation of sensory precursors in the postnatal mouse cochlea.
Proc Natl Acad Sci U S A
May 2012
Department of Otolaryngology-Head and Neck Surgery, Stanford University School of Medicine, Stanford, CA 94305, USA.
Inner ear hair cells are specialized sensory cells essential for auditory function. Previous studies have shown that the sensory epithelium is postmitotic, but it harbors cells that can behave as progenitor cells in vitro, including the ability to form new hair cells. Lgr5, a Wnt target gene, marks distinct supporting cell types in the neonatal cochlea.
View Article and Find Full Text PDFStereocilia-like structures in the endolymphatic sac in Ménière's disease and acoustic neuroma.
ORL J Otorhinolaryngol Relat Spec
March 1999
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