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Using uniparental genetic profiles to unravel the complexity of Argentine admixed populations.
Forensic Sci Int Genet
December 2024
DNA Diagnostic Laboratory, Institute of Biology Roberto Alcantara Gomes, State University of Rio de, Rio de Janeiro 20550-900, Brazil.
Latin American countries are distinguished by their highly admixed populations, characterized by a significant preservation of Native American matrilineal ancestry. This contrasts with the paternal lineages, which exhibit different patterns due to pronounced sex-biased mating practices during the colonial period. Uniparental genetic markers have been instrumental in population genetics, facilitating the reconstruction of human settlement histories and serving forensic identification purposes.
View Article and Find Full Text PDFComparison of Plant Genomic DNA Extraction Kits Using the Proofman-LMTIA Amplification Assay.
Phytochem Anal
December 2024
School of Food and Biological Engineering, Henan University of Science and Technology, Luoyang, Henan, China.
Introduction: The extraction of DNA is the basis of molecular biology research. The quality of the extracted DNA is one of the key factors for the success of molecular biology experiments.
Objective: To select a suitable DNA extraction method for Chinese medicinal herbs and seeds.
Detecting epithelial-mesenchymal transition signaling molecules in cervical epithelial cells aids in the early diagnosis of cervical lesions.
BMC Cancer
December 2024
Department of Laboratory Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, 510515, P. R. China.
Background: This study aimed to investigate the potential utility of Epithelial-mesenchymal transition (EMT) signaling cell detection in the early diagnosis of cervical lesions.
Methods: Enrichment of cervical epithelial cells was carried out using a calibrated membrane with 8-μm diameter pores. RNA-in situ hybridization (RNA-ISH) was employed to detect and characterize EMT cells utilizing specific EMT markers.
Novel isothermal nucleic acid amplification method for detecting malaria parasites.
Appl Microbiol Biotechnol
December 2024
Laboratório de Pesquisa em Malária, Instituto Oswaldo Cruz (IOC), Fundação Oswaldo Cruz (Fiocruz), Rio de Janeiro, Brazil.
Malaria, a parasitic disease caused by Plasmodium spp. and transmitted by Anopheles mosquitoes, remains a major global health issue, with an estimated 249 million cases and 608,000 deaths in 2022. Rapid and accurate diagnosis and treatment are crucial for malaria control and elimination.
View Article and Find Full Text PDFProof of Concept Study: Comparison of Semi-Automated RNA Isolation Methods from Archived Formalin-Fixed, Paraffin-Embedded Tissues with Clinical Routine RNA Isolation Methods.
Methods Protoc
December 2024
Department of Pathology, Herlev University Hospital, 2730 Herlev, Denmark.
High-quality RNA is crucial in clinical diagnostics and precision medicine. Formalin-fixed and paraffin-embedded (FFPE) tissues pose a challenge due to nucleic acid fragmentation and crosslinking. In this pilot study, various commercially available techniques for extracting RNA from small FFPE samples were compared.
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