AI Article Synopsis
- Electric field pulses can enhance the permeability of cell membranes, allowing for the introduction of various substances into cells.
- Low molecular weight fluorescent probes and the high molecular weight enzyme pronase, which mimics insulin effects, were successfully loaded into intact rabbit erythrocytes.
- This method resulted in effective loading with high cell survival rates, suggesting potential clinical applications for diagnosis and therapy.
Article Abstract
The application of electric field pulses in cell suspensions is known to alter membrane integrity, resulting in increased membrane permeabilization. This field-induced membrane poration provides the means to load cells with a variety of external substances, useful for clinical applications. In this work, intact rabbit erythrocytes were successfully loaded with low molecular weight fluorescent probes and with the high molecular weight enzyme pronase, which has been shown to mimic the effects of insulin. Attachment of the enzyme onto the cell surface was also achieved by modifying the applied pulse parameters. Both applications were efficient and accompanied by high cell survival rates. In this way, biological carriers loaded with active substances were produced, offering the potentials for useful clinical applications, either for diagnostic or therapeutic purposes.
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