In experiments with a primary culture of adrenal cells, it has been studied the influence of chloditane, as a specific inhibitor of adrenocortical function, on the synthetic processes in the newborn pig adrenal cells, whose organ and cell cultures are more and more used as transplantation material for the treatment of adrenal insufficiency. It has been shown that incubation of adrenocorticocytes with chloditane (final concentration in culture medium: 1 to 10,000 ng/ml) during 24 hours had a marked negative dose-dependent effect on the indices of inclusion of 3H-thymidine and 3H-uridine into the nucleic acids. In the presence of a maximum chloditane concentration, inclusion made, respectively, 16.9 and 46.0% from control. A trustworthy effect of chloditane on the indices of 3H-leucine inclusion (protein synthesis) has been established only for concentration 10 to 100 ng/ml and made near 70% from control. It has been discussed a possible mechanism of chloditane action on the synthetic processes in adrenal cells.
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