The presence of desmin is used to identify the Ito cells in rat liver and to evaluate the purity of separated and cultured cells. The present study was conducted to evaluate the expression of cytoskeletal (with special emphasis on desmin) and intercellular matrix proteins in cultured rat liver Ito cells. The Ito cells were isolated by enzymatic digestion of rat liver and purified by single-step Nycodenz gradient. During the first week of culturing, the Ito cells proliferated and all of them expressed desmin and vimentin. By the end of the first week, part of them were seen to transdifferentiate into myofibroblasts. These cells expressed alpha-smooth muscle actin. The Ito cells and myofibroblasts were stained with anti-laminin, and anti-collagen I and III antibodies. The expression of desmin in myofibroblasts was less than in untransformed (stellate shaped) Ito cells. By the end of the second week the cells developed a dense monolayer and lost desmin. We suggest that repression of desmin in the cablestone-like monolayer of cells may be due to cell-to-cell contact inhibition.
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ACS Appl Mater Interfaces
January 2025
CSIR-National Chemical Laboratory, Dr. Homi Bhabha Road, Pune 411008, India.
The tunability of the energy bandgap in the near-infrared (NIR) range uniquely positions colloidal lead sulfide (PbS) quantum dots (QDs) as a versatile material to enhance the performance of existing perovskite and silicon solar cells in tandem architectures. The desired narrow bandgap (NBG) PbS QDs exhibit polar (111) and nonpolar (100) terminal facets, making effective surface passivation through ligand engineering highly challenging. Despite recent breakthroughs in surface ligand engineering, NBG PbS QDs suffer from uncontrolled agglomeration in solid films, leading to increased energy disorder and trap formation.
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January 2025
Department of Materials Science and Engineering, City University of Hong Kong, Kowloon, Hong Kong, 999077, China.
Carbazole-derived self-assembled monolayers (SAMs) are promising materials for hole-extraction layer (HEL) in conventional organic photovoltaics (OPVs). Here, a SAM Cbz-2Ph derived from 3,6-diphenylcarbazole is demonstrated. The large molecular dipole moment of Cbz-2Ph allows the modulation of electrode work function to facilitate hole extraction and maximize photovoltage, thus improving the OPV performance.
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January 2025
Shaanxi Key Laboratory of Degradable Biomedical Materials, Shaanxi R&D Center of Biomaterials and Fermentation Engineering, Biotech & Biomed Research Institute, School of Chemical Engineering, Northwest University, Xi'an, China.
Liver disease constitutes a significant cause of global mortality, with its pathogenesis being multifaceted. Identifying effective pharmacological and preventive strategies is imperative for liver protection. Ginsenosides, the major bioactive compounds found in ginseng, exhibit multiple pharmacological activities including protection against liver-related diseases by mitigating liver fat accumulation and inflammation, preventing hepatic fibrosis, and exerting anti-hepatocarcinogenic effects.
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January 2025
College of Korean Medicine, Gachon University, Seongnam, 13120, South Korea. Electronic address:
Obesity due to excessive body fat accumulation remains a global problem. Patients with obesity have high cortisol levels, and its dysregulation is caused by increased 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) levels. The effects and mechanism of J2H-1702, an 11β-HSD1 inhibitor, on nonalcoholic steatohepatitis (NASH) were explored.
View Article and Find Full Text PDFJ Colloid Interface Sci
January 2025
Department of Pharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang, Liaoning Province 110016, PR China. Electronic address:
Liver fibrosis is a common pathological stage in the development of several chronic liver diseases, and early intervention can effectively reverse the developing process. Excessive reactive oxygen species (ROS) can promote the activation of hepatic stellate cells (HSCs), but existing treatments have not addressed this problem. In this study, different metal-based mesoporous polydopamine (MPDA) was prepared by the soft template method, and their free radical scavenging abilities, as well as the efficacy and safety of the carriers were investigated, so as to select Cu-coordinated MPDA (CMP) as the optimal nanocarrier.
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