Hypermedia, animation and color coding were used to develop a module to visualize the lymphatic drainage of the cerebrospinal fluid and the research procedures that quantitate this drainage, to help researchers present their findings to a variety of audiences. Basic and clinical science evaluators found the method successful. Suggested uses for the module included teaching, conference and Web presentation, and liaising with research sponsors. Respondents emphasized that researchers need private investment to afford the cost of hypermedia modules. A thorough literature review, formative feedback, and post-evaluation proved fundamental to the development of this hypermedia program.
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Alzheimers Dement
December 2024
Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore, Singapore.
Background: Perivascular spaces (PVS) are fluid-filled spaces in the brain, hypothesized in promoting clearance of metabolites implicated in dementia through glymphatic system drainage. PVS are classified according to Grades (0-4). Blood-based biomarkers including Glial fibrillary acidic protein (GFAP), Amyloid β 42/40 (Aβ42/40) ratio have shown promise in diagnosing and prognosing dementia.
View Article and Find Full Text PDFAlzheimers Dement
December 2024
Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Background: As blood-based biomarkers become critical to Alzheimer's Disease (AD) clinical testing, establishment of less invasive plasma collection methods are key. We compared the Tasso+ and the TAPII capillary whole blood collection devices to traditional venipuncture for safety and efficacy in our clinic and measured AD biomarkers neurofilament-light (NFL), glial fibrillary acidic protein (GFAP), phosphorylated-tau 217 (ptau217), and amyloid-beta 42/40 ratio (Aβ42/40) in cerebrospinal fluid (CSF), capillary, and venous plasma to assess potential age and cognition related group differences.
Method: Patients seen at the Johns Hopkins Center for CSF Disorders (N = 193) underwent diagnostic lumbar punctures and/or extended lumbar drainage procedures.
J Exp Med
February 2025
Brain Immunology and Glia (BIG) Center, Washington University in St. Louis, St. Louis, MO, USA.
Dysfunctional lymphatic drainage from the central nervous system (CNS) has been linked to neuroinflammatory and neurodegenerative disorders, but our understanding of the lymphatic contribution to CNS fluid autoregulation remains limited. Here, we studied forces that drive the outflow of the cerebrospinal fluid (CSF) into the deep and superficial cervical lymph nodes (dcLN and scLN) and tested how the blockade of lymphatic networks affects CNS fluid homeostasis. Outflow to the dcLN occurred spontaneously in the absence of lymphatic pumping and was coupled to intracranial pressure (ICP), whereas scLN drainage was driven by pumping.
View Article and Find Full Text PDFAntibiotics (Basel)
December 2024
Infectious Diseases Unit, Trieste University Hospital (ASUGI), 34125 Trieste, Italy.
: The early identification of infection-causing microorganisms through multiplex PCR panels enables prompt and targeted antibiotic therapy. This study aimed to assess the performance of the BioFire Joint Infection Panel (BF-JIP) in analysing non-synovial fluid samples. : We conducted a retrospective cohort study at Trieste University Hospital, Italy, on hospitalised adults with non-synovial fluid samples tested by both BF-JIP and traditional culture methods (November 2022-April 2024).
View Article and Find Full Text PDFBrain Sci
December 2024
School of Mechanical Engineering, University of New South Wales, Sydney, NSW 2052, Australia.
Background/objectives: Cerebrospinal infusion studies indicate that cerebrospinal fluid outflow resistance (R) is elevated in normal pressure hydrocephalus (NPH). These studies assume that the cerebrospinal formation rate (CSF) does not vary during the infusion. If the CSF were to increase during the infusion then the R would be overestimated.
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