AI Article Synopsis
- - A new micromethod was created that uses a protein-dye binding reaction on a special acetate membrane to measure tiny protein amounts in limited biological samples.
- - The method involves discoidal cellulose acetate membranes that can absorb a very small protein solution (0.2-2.0 micrograms) and are stained with Coomassie Brilliant Blue G-250.
- - When examined under a light microscope with an automatic exposure instrument, the study found a direct correlation between the dye’s binding intensity to protein and the exposure time, under certain optical conditions.
Article Abstract
A micromethod which utilizes a protein-dye binding reaction on an acetate membrane and a light microscope with an automatic exposure instrument has been developed for measuring small amounts of protein from limited biological materials. After discoidal cellulose acetate membranes (2.0 mm diameter), which absorbed 0.5 microliter of protein solution containing 0.2-2.0 micrograms of protein, were stained with Coomassie Brilliant Blue G-250, they were examined by a light microscope with an automatic exposure instrument. A linear relation was confirmed between the intensity of dye binding to protein and exposure time under specific optical conditions.
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| http://dx.doi.org/10.1292/jvms.60.1141 | DOI Listing |
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