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Global Prevalence of Neospora caninum in Domestic Pigs (Sus domesticus) and Wild Boars (Sus scrofa): A Systematic Review and Meta-Analysis.
Vet Med Sci
January 2025
Medical Biology Research Center, Health Technology institute, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Background: Neospora caninum (Apicomplexa, Sarcocystidae) is a protozoan parasite regarded as a major cause of reproductive failure in cattle. Swine are susceptible to N. caninum infection; however, the role of these animals in the circulation, maintenance, and transmission of N.
View Article and Find Full Text PDFOptimization of a Conventional Assay into SYBR Green Real-Time PCR for the Detection of the Nc5 Segment from Neospora caninum.
Acta Parasitol
January 2025
Department of Veterinary Medicine, Federal University of Paraná, Rua Dos Funcionários, 1540, Curitiba, Paraná, 80035-050, Brazil.
Purpose: The aim of the present study was to establish a SYBR Green-based real-time PCR assay for detection of the Nc5 segment from the Neospora caninum genome.
Methods: The oligonucleotides sequences targeting the Nc5 gene previously reported and designed in-house were validated. Two Primer sets were evaluated and tested in four different combinations.
Bovine PMN responses to extracellular vesicles released by tachyzoites and -infected host cells.
Front Immunol
January 2025
Institute of Parasitology, Justus Liebig University Giessen, Giessen, Germany.
Bovine besnoitiosis is a re-emerging cattle disease caused by the apicomplexan parasite , which severely affects individual animal welfare and profitability in cattle industry. We recently showed that tachyzoite exposure to bovine polymorphonuclear neutrophils (PMN) effectively triggers neutrophil extracellular trap (NET) formation, leading to parasite immobilization hampering host cell infection. So far, the triggers of this defense mechanism remain unclear.
View Article and Find Full Text PDFSeroprevalence of bovine Herpesvirus-1, bovine viral diarrhoea virus, Neospora caninum and Coxiella burnetii in dairy cows in Ethiopia.
BMC Res Notes
December 2024
Ethiopian Institute of Agricultural Research, National Agricultural Biotechnology Research Center, P.O. Box: 249, Holeta, Ethiopia.
Background: The reproductive problem is an animal health-related bottleneck that constrains livestock genetic improvement efforts in tropical countries such as Ethiopia. The infectious causes of reproductive disorders are one cause of decreased reproductive efficiency. This study aimed to determine the seroprevalence to Bovine Herpesvirus-1 (BHV1), Bovine Viral Diarrhea Virus (BVDV), Neospora caninum (N.
View Article and Find Full Text PDFMorphological and molecular characterization of a Sarcocystis bovifelis-like sarcocyst in American beef.
Parasit Vectors
December 2024
United States Department of Agriculture, Agricultural Research Service, Beltsville Agricultural Research Centre, Animal Parasitic Diseases Laboratory, Beltsville, MD, 20705-2350, USA.
Background: Parasites in the apicomplexan genus Sarcocystis infect cattle worldwide. Assessing the economic importance of each such parasite species requires proper diagnosis. Sarcocystis cruzi, a thin-walled species, infects virtually all cattle.
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