The monoclonal antibody-specific immobilization of platelet antigens (MAIPA) assay is considered as a possible reference method for the detection of maternal alloantibodies when a foetomaternal alloimmunization is suspected. However, this method is tedious. In this study, we have compared the MAIPA results of 54 samples of mothers with (n=34) or without (n=20) alloantibodies with those obtained with a new antigen capture ELISA. Using the cutoff of 2.0 given by the manufacturer, the new kit had a sensitivity of 88.2% (95% CI 72.6-96.7) and a specificity of 100% (95% CI 98.0-100). From a receiver-operating characteristic curve analysis, the more appropriate cutoff for a screening assay would be 1.6, which gives a sensitivity of 100% (95% CI 89.7-100) with a specificity of 94.0% (95% CI 89.4-96.9). In conclusion, this new simple assay appears promising and could be used, with the modified cutoff, as a screening assay for the detection of platelet alloantibodies.
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