Effect of DNA-intercalators ethidium bromide (EB, 0.005 and 0.015 mM) and dimidium bromide (DB, 0.005 and 0.010 mM) and antioxidative compounds acetylsalicylic acid (ASA, 0.05 and 0.50 mM) and beta-carotene (0.01, 0.02, 0.05 mM) on the phagosome-lysosome (P-L) fusion and F-actin content in murine peritoneal macrophages were studied. EB, DB, ASA and beta-carotene were found to stimulate P-L fusion and the effect depending on the concentration of compounds tested. The strongest influence as evoked by 0.5 mM of ASA and 0.05 mM of beta-carotene. The compounds tested enhanced the F-actin content in macrophages, especially by the action of beta-carotene (0.05 mM). The obtained data indicate a correlation between P-L fusion stimulation and F-actin content under the influence of compounds tested in murine peritoneal macropheages.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Two ligands of Arp2/3 complex, yeast coronin and GMF, interact and synergize in pruning branched actin networks.
J Biol Chem
January 2025
Department of Biology, Rosenstiel Basic Medical Science Research Center, Brandeis University, Waltham, MA, USA. Electronic address:
The rapid turnover of branched actin networks underlies key in vivo processes such as lamellipodial extension, endocytosis, phagocytosis, and intracellular transport. However, our understanding of the mechanisms used to dissociate, or 'prune', branched filaments has remained limited. Glia maturation factor (GMF) is a cofilin family protein that binds to Arp2/3 complex and catalyzes branch dissociation.
View Article and Find Full Text PDFHighly Flexible Methyl Cellulose/Gelatin Hydrogels for Potential Cartilage Tissue Engineering Applications.
Biopolymers
January 2025
Institute of Biomedical Engineering, Boğaziçi University, Istanbul, Turkey.
Cartilage damage resulting from trauma demonstrates a poor capacity for repair due to its avascular nature. Cartilage tissue engineering offers a unique therapeutic option for cartilage recovery. In this study, methylcellulose (MC)/gelatin (GEL) hydrogels (MC10G20, MC12.
View Article and Find Full Text PDFCoenzyme Q10 Alleviates Silicosis Fibrosis Inhibiting Ferroptosis in Mice.
In Vivo
December 2024
College of Biology, Hunan University, Changsha, P.R. China;
Background/aim: Silicosis, the most severe type of occupational pneumoconiosis, leads to diffuse pulmonary fibrosis without specific therapy. Ferroptosis is triggered by reactive oxygen species (ROS) and Fe overload-induced lipid peroxidation, which is involved in the progression of pulmonary fibrosis. As an important coenzyme in the process of aerobic respiration, Coenzyme Q10 (CoQ10) can enhance mitochondrial function and energy supply and reduce malondialdehyde (MDA) to limit the risk of fibrosis.
View Article and Find Full Text PDFGuilu Erxian Jiao remodels dendritic spine morphology through activation of the hippocampal TRPC6-CaMKIV-CREB signaling pathway and suppresses fear memory generalization in rats with post-traumatic stress disorder.
J Ethnopharmacol
January 2025
Integrative Medicine Research Center, School of Basic Medical Sciences, Guangzhou University of Chinese Medicine, Outer Ring East Road No. 232, Higher Education Mega Center, Guangzhou, 510006, China. Electronic address:
Ethnopharmacological Relevance: Guilu Erxian Jiao (GLEXJ) is a renowned traditional Chinese herbal formula used to tonify the kidney. It is employed to treat psychiatric disorders, and alleviate memory impairment, cognitive dysfunction, and behavioral disorders. Modern pharmacological studies have demonstrated GLEXJ's ability to significantly inhibit the fear response in post-traumatic stress disorder (PTSD) and facilitate the extinction of fear memory.
View Article and Find Full Text PDFThe mode of subunit addition regulates the processive elongation of actin filaments by formin.
J Biol Chem
January 2025
Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, Minnesota, USA. Electronic address:
Article Synopsis
- Formins are key proteins that promote actin filament growth by binding to the barbed ends and facilitating the addition of actin subunits through their unique FH2 and FH1 domains.
- Research on the yeast formin Bni1p showed that filament length is influenced by the elongation rate and the probability of formin dissociation, which varies under different conditions.
- The study highlights the important role of FH1 domains in controlling the processivity of formins, affecting the way filaments are assembled and their average lengths, tailored to the specific needs of the cell.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!