CATT/Trypanosoma brucei (T.b.) gambiense is an antibody detection test currently used in field surveys on Gambian sleeping sickness. The screening test is usually performed on a drop of freshly collected heparinized blood, followed by a more specific confirmation test on diluted blood, plasma or serum. This approach may be biased by the occurrence of a complement-mediated prozone phenomenon causing lower test sensitivity at lower sample dilutions. A simple remedy is by addition of a Ca2+ chelating agent such as EDTA.
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Identification of human African Trypanosomiasis foci using school-going children in post-conflict era in Nwoya District, Northern Uganda: A cross-sectional study.
AAS Open Res
April 2018
Department of Medical Biochemistry, Faculty of Medicine, Gulu University, Gulu, Uganda.
Human African Trypanosomiasis (HAT) is fatal if untreated; the drugs to treat it are toxic making its management difficult and diagnosis complex. Nwoya district has a long history of sleeping-sickness dating back to pre-colonial times. The civil war of 1986-2008 displaced many who upon return complained of cattle and dogs dying of unknown causes alongside increased tsetse flies infestation hence, the needs for the study.
View Article and Find Full Text PDFSensitivity and specificity of HAT Sero-K-SeT, a rapid diagnostic test for serodiagnosis of sleeping sickness caused by Trypanosoma brucei gambiense: a case-control study.
Lancet Glob Health
June 2014
Institute of Tropical Medicine, Department of Biomedical Sciences, Antwerp, Belgium; Institut de Recherche pour le Développement, UMR 177, Montpellier, France.
Background: Human African trypanosomiasis (HAT) is a life-threatening infection affecting rural populations in sub-Saharan Africa. Large-scale population screening by antibody detection with the Card Agglutination Test for Trypanosomiasis (CATT)/Trypanosoma brucei (T b) gambiense helped reduce the number of reported cases of gambiense HAT to fewer than 10 000 in 2011. Because low case numbers lead to decreased cost-effectiveness of such active screening, we aimed to assess diagnostic accuracy of a rapid serodiagnostic test (HAT Sero-K-SeT) applicable in primary health-care centres.
View Article and Find Full Text PDFEvaluation of the micro-CATT, CATT/Trypanosoma brucei gambiense, and LATEX/T b gambiense methods for serodiagnosis and surveillance of human African trypanosomiasis in West and Central Africa.
Bull World Health Organ
February 2003
Institut de Recherche pour le Développement, Organisation de Coordination pour la Lutte contre les Endémies en Afrique Centrale, Yaoundé, Cameroon.
Objective: To evaluate the performance of serological tests using dried blood on filter-papers (micro-card agglutination test for trypanosomiasis (micro-CATT)) performed under field and laboratory conditions and using whole blood ((CATT/T.b. gambiense) (wb-CATT) and latex agglutination (LATEX/T.
View Article and Find Full Text PDFField evaluation of the CATT/Trypanosoma brucei gambiense on blood-impregnated filter papers for diagnosis of human African trypanosomiasis in southern Sudan.
Trop Med Int Health
November 2002
Médecins Sans Frontières (MSF) - Switzerland, Geneva, Switzerland.
Most Human African Trypanosomiasis (HAT) control programmes in areas endemic for Trypanosoma brucei gambiense rely on a strategy of active mass screening with the Card Agglutination Test for Trypanosomiasis (CATT)/T. b. gambiense.
View Article and Find Full Text PDFEvaluation of an EDTA version of CATT/Trypanosoma brucei gambiense for serological screening of human blood samples.
Acta Trop
January 2002
Department of Parasitology, Prince Leopold Institute of Tropical Medicine, Nationalestraat 155, B-2000, Antwerp, Belgium.
CATT/Trypanosoma brucei gambiense, a direct card agglutination test designed for field surveys on human African trypanosomosis, is currently used with freshly collected heparinized blood samples. When testing serum samples, it has been observed earlier that, at lower sample dilutions, a complement-mediated inhibition phenomenon may cause false negative test results. This can be avoided by adding an anticomplementary agent such as di-sodium ethylenediaminetetraacetate dihydrate (EDTA) to the reaction.
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