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Argonaute-mediated dual recognition enables the detection of trace single-nucleotide-mutated fungicide-resistant fungal pathogens.
Chem Commun (Camb)
January 2025
Beijing Life Science Academy, Beijing 102206, China.
Detection of low-abundance mutations for the early discovery of fungicide-resistant fungal pathogens is highly demanded, but remains challenging. Herein, we developed a dual-recognition strategy, termed PARPA, involving s Argonaute (pfAgo)-mediated elimination of wild-type fungal genes and CRISPR/Cas12a-based amplicon recognition. This assay can detect fungicide-resistant at relative abundances as low as 0.
View Article and Find Full Text PDFMechanisms for removing phosphorus species through sequential coagulation using inorganic coagulants and organic polymers.
Water Sci Technol
January 2025
Norwegian University of Life Sciences, Department of Building and Environmental Technology, P.O. Box 5003, 1430 Ås, Norway.
The need for stringent phosphorus removal from domestic wastewater is increasing to mitigate eutrophication, while efficient phosphate reuse is critical due to the global phosphate crisis. Combining aluminum sulfate (ALS) with high molecular weight organic polymers achieved 95-99% removal of particles, turbidity, and phosphates, reducing ALS usage by 40%. We propose mechanisms to explain the enhanced treatment efficiency.
View Article and Find Full Text PDFThe field of π-conjugated organic materials has seen significant advances in recent years. However, enhancing the functionality of well-established, mass-produced compounds remains a considerable challenge, despite being an intriguing strategy for designing high-value organic materials with low production costs. In this context, vat dyes, known for their wide range of colors and extensive use in the textile industry are particularly attractive.
View Article and Find Full Text PDFCharacterizing the microbiome recruited by the endangered plant in phosphorus-deficient acidic soil.
Front Microbiol
January 2025
Hawkesbury Institute for the Environment, Western Sydney University, Penrith, NSW, Australia.
Phosphorus (P)-deficient soils serve as crucial habitats for endangered plant species. Microbiomes play pivotal roles in soil element cycling and in determining a plant's adaptability to the environment. However, the relationship between the endangered plant, microbiome, and soil stoichiometric traits, and how it affects plant adaption to P-deficient habitats remain largely unexplored.
View Article and Find Full Text PDFUnveiling microbial diversity in slightly and moderately magnesium deficient acidic soils.
Sci Rep
January 2025
Department of Agricultural Microbiology, Tamil Nadu Agricultural University, Coimbatore, 641003, Tamil Nadu, India.
Magnesium (Mg) an essential plant nutrient is widespread deficient in the acidic soils of Nilgiris of Tamil nadu, India. The vegetable yield and quality is especially affected due to deficiency of nutrients like Mg. This study investigates soil characteristics and bacterial diversity in the Nilgiris district of Tamil Nadu, India, with respect to Mg deficiency.
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