A new restriction endonuclease Abe I has beenisolated from Azotobacter beijerinckii. This enzymerecognizes the asymmetric heptanucleotide sequence 5'-CCTCAGC-3' and cleaves within it symmetrically at positions -5/-2 in the opposing strands, producing three base protruding 5'-ends.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC147928PMC
http://dx.doi.org/10.1093/nar/26.21.4917DOI Listing

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