Degenerate primers and the polymerase chain reaction (PCR) detected a conserved region of copia-like reverse transcriptase from Anopheles mosquitoes in Thailand. A total of 43 subclone PCR fragments of the size expected for reverse transcriptase of copia-like elements was isolated from Anopheles dirus (Peyton & Harrison) subspecies A, Anopheles maculatus (Theobald) subspecies E, Anopheles nivipes (Theobald), and Anopheles subpictus Grassi. Sequence analysis of subclones confirmed the identity of these sequences as copia-like reverse transcriptase sequences. The sequences displayed varying degrees of sequence heterogeneity, in contrast to the limited diversity seen in copia-like elements in Drosophila. Phylogenetic analysis of the amino acid sequences of the subclones showed that the majority of the retroelements were clustered together, implying that sequence divergence during vertical transmission of the copia-like retrotransposons has been a major factor in the evolution of copia-like retroelements in Anopheles species. Additionally, there is evidence that horizontal transfer of this transposon group among certain divergent taxa also may have played a role in their evolution.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1093/jmedent/35.5.771 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Learning From Full Characterization of HIV Proviruses in People Receiving Long-Acting Cabotegravir/Rilpivirine With a History of Replication on the Antiretroviral Classes.
Open Forum Infect Dis
January 2025
CHU d'Orléans, Orléans, France.
Background: To better understand factors associated with virologic response, we retrospectively characterized the HIV proviruses of 7 people with HIV who received long-acting cabotegravir/rilpivirine (CAB/RPV-LA) and were selected according to the following criteria: virologic control achieved despite a history of viral replication on 1 or both corresponding antiretroviral classes (n = 6) and virologic failure (VF) after CAB/RPV-LA initiation (n = 1).
Methods: Last available blood samples before the initiation of CAB/RPV-LA were analyzed retrospectively. Near full-length HIV DNA genome haplotypes were inferred from Nanopore sequencing by the in vivo Genome Diversity Analyzer to search for archived drug resistance mutations (DRMs) and evaluate the frequency and intactness of proviruses harboring DRMs.
Since the emergence of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), the need for an effective vaccine has appeared crucial for stimulating immune system responses to produce humoral/cellular immunity and activate immunological memory. It has been demonstrated that SARS-CoV-2 variants escape neutralizing immunity elicited by previous infection and/or vaccination, leading to new infection waves and cases of reinfection. The study aims to gain into cases of reinfections, particularly infections and/or vaccination-induced protection.
View Article and Find Full Text PDFObstacles in quantifying A-to-I RNA editing by Sanger sequencing.
Methods Enzymol
January 2025
Faculty of Biology, Technion - Israel Institute of Technology, Technion City, Haifa, Israel. Electronic address:
Adenosine-to-Inosine (A-to-I) RNA editing is the most prevalent type of RNA editing, in which adenosine within a completely or largely double-stranded RNA (dsRNA) is converted to inosine by deamination. RNA editing was shown to be involved in many neurological diseases and cancer; therefore, detection of A-to-I RNA editing and quantitation of editing levels are necessary for both basic and clinical biomedical research. While high-throughput sequencing (HTS) is widely used for global detection of editing events, Sanger sequencing is the method of choice for precise characterization of editing site clusters (hyper-editing) and for comparing levels of editing at a particular site under different environmental conditions, developmental stages, genetic backgrounds, or disease states.
View Article and Find Full Text PDFNanopore sequencing to detect A-to-I editing sites.
Methods Enzymol
January 2025
School of Chemistry, Chemical Engineering and Biotechnology, Nanyang Technological University, Singapore, Singapore. Electronic address:
Adenosine-to-inosine (A-to-I) RNA editing, mediated by the ADAR family of enzymes, is pervasive in metazoans and functions as an important mechanism to diversify the proteome and control gene expression. Over the years, there have been multiple efforts to comprehensively map the editing landscape in different organisms and in different disease states. As inosine (I) is recognized largely as guanosine (G) by cellular machineries including the reverse transcriptase, editing sites can be detected as A-to-G changes during sequencing of complementary DNA (cDNA).
View Article and Find Full Text PDFEffectiveness of Frequent Point-of-Care Molecular COVID-19 Surveillance in a Rural Workplace: Nonrandomized Controlled Clinical Trial Among Miners.
JMIR Public Health Surveill
January 2025
Center for Global Health, University of New Mexico Health Sciences Center, Albuquerque, NM, United States.
Background: Numerous studies have assessed the risk of SARS-CoV-2 exposure and infection among health care workers during the pandemic. However, far fewer studies have investigated the impact of SARS-CoV-2 on essential workers in other sectors. Moreover, guidance for maintaining a safely operating workplace in sectors outside of health care remains limited.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!