Dogs were investigated to determine if they are definitive hosts of Neospora caninum. Four dogs were fed N. caninum tissue cysts in infected mouse tissue, and two negative control dogs were fed uninfected mouse tissue. Dog faeces were examined daily for 30 days using a sucrose flotation technique. Three challenged dogs shed spherical to subspherical unsporulated oocysts, measuring 10 to 11 microns in diameter. Oocysts sporulated within 3 days and contained two sporocysts, each with four sporozoites. Outbred, inbred, and gamma-interferon knockout mice were inoculated with canine faecal extracts and monitored for evidence of neosporosis using a variety of morphologic, immunohistologic, serologic, and genetic analyses. Mice that received faeces from each dog observed to shed oocysts were demonstrated to have neosporosis by two or more techniques. One mouse was demonstrated to be infected with N. caninum by immunohistochemistry, ultrastructural analysis, and a species-specific PCR test. No evidence of neosporosis was observed in control animals. Based on this study, dogs are a definitive host of Neospora caninum.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Optimization of a Conventional Assay into SYBR Green Real-Time PCR for the Detection of the Nc5 Segment from Neospora caninum.
Acta Parasitol
January 2025
Department of Veterinary Medicine, Federal University of Paraná, Rua Dos Funcionários, 1540, Curitiba, Paraná, 80035-050, Brazil.
Purpose: The aim of the present study was to establish a SYBR Green-based real-time PCR assay for detection of the Nc5 segment from the Neospora caninum genome.
Methods: The oligonucleotides sequences targeting the Nc5 gene previously reported and designed in-house were validated. Two Primer sets were evaluated and tested in four different combinations.
Seroprevalence of bovine Herpesvirus-1, bovine viral diarrhoea virus, Neospora caninum and Coxiella burnetii in dairy cows in Ethiopia.
BMC Res Notes
December 2024
Ethiopian Institute of Agricultural Research, National Agricultural Biotechnology Research Center, P.O. Box: 249, Holeta, Ethiopia.
Background: The reproductive problem is an animal health-related bottleneck that constrains livestock genetic improvement efforts in tropical countries such as Ethiopia. The infectious causes of reproductive disorders are one cause of decreased reproductive efficiency. This study aimed to determine the seroprevalence to Bovine Herpesvirus-1 (BHV1), Bovine Viral Diarrhea Virus (BVDV), Neospora caninum (N.
View Article and Find Full Text PDFEpidemiology of Bovine Neosporosis in Relation to Socioeconomic, Demographic, and Transmissibility Factors in Dual-Purpose Production Systems in Colombia.
Epidemiologia (Basel)
December 2024
Centro de Investigación Turipaná, Corporación Colombiana de Investigación Agropecuaria AGROSAVIA, km 13 vía Montería, Cereté 230550, Colombia.
Introduction: Bovine neosporosis represents a significant threat to reproduction and production in livestock systems worldwide. This disease is caused by the protozoan , resulting in abortions of cows and neurological signs in newborn calves. This leads to significant economic losses, decreasing meat and milk production, especially in tropical regions.
View Article and Find Full Text PDFSeroprevalence and factors associated with exposure to among dairy cattle smallholders in southern Rio Grande do Sul.
Braz J Vet Med
December 2024
Veterinarian, Departamento de Veterinária Preventiva, Universidade Federal de Pelotas, Pelotas, RS, Brazil.
is a protozoan parasite that infects several species of animals (domestic and wild) and is one of the most common causes of abortion in cattle worldwide. To better understand the epidemiological chain of neosporosis, update the disease status and propose control measures to improve milk production in Rio Grande do Sul (RS), the present study aimed to evaluate the seroprevalence of and its distribution in different municipalities of Rio Grande do Sul, Southern RS, Brazil, and determine the factors associated with exposure to in small dairy cattle producers in this region. Cattle from 51 dairy farms located in nine municipalities in the southern region of RS were included in this study.
View Article and Find Full Text PDFShedding light on risk: Seroprevalence of Q fever among farm animals and workers in Ecuador.
One Health
December 2024
One Health Research Group, Facultad de Ciencias de la Salud, Universidad de Las Américas, Quito, Ecuador, 170503.
Q fever, caused by the bacterium , is a zoonotic disease that has been largely overlooked despite presenting significant risks to both animal and public health. Although well studied in some countries, in most countries in Latin America, there's a lack of information on infection, its prevalence, and its impact on both livestock and human populations. To address this gap, we conducted a serosurvey among farm workers, cattle, sheep, and dogs on two dairy farms in Ecuador using a commercial ELISA kit.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!