The ParaSight F is a new diagnostic test for Plasmodium falciparum infections and is based on the detection of a trophozoite-derived antigen, the histidine rich protein II (HRP-II). To assess the usefulness of this test, we conducted a prospective study and analyzed 62 blood specimens from 38 patients, using thin blood films, thick blood films and the ParaSight F test. Compared to thick blood film, on samples taken before and during treatment, the ParaSight F test had 86.4% sensitivity and 100% specificity. In 31.5% of P. falciparum infected patients, parasitemia was lower than 1 parasite/1000 red blood cells, with all specimens being positive by the ParaSight F test. In 15 cases, specimens were negative by thin blood film, but were positive by thick blood film and by the ParaSight F test. Two patients had, after their treatment was started, positive results by ParaSight F and negative results by thick blood film. Cross-reactivity occurred neither with other Plasmodium species, nor in cases of severe inflammatory syndrome. Persistence of antigenemia was monitored in 14 patients receiving quinine. At day five of treatment, antigenemia persisted in seven patients. In conclusion, the ParaSight F test does not allow following up the efficacy of treatment, identifying other Plasmodium species, or assessing parasitemia. However, because this test is easy to perform and has good sensitivity and specificity, it is a useful tool in emergent context, in cases of parasitemia lower than the thin blood film threshold, and in cases morphologically difficult to decipher.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Evaluation of Parasight All-in-One system for the automated enumeration of helminth ova in canine and feline feces.
Parasit Vectors
June 2024
Parasight System Inc., Suite 2130, 1532 N. Limestone St., Lexington, KY, 40505, USA.
Background: Digital imaging combined with deep-learning-based computational image analysis is a growing area in medical diagnostics, including parasitology, where a number of automated analytical devices have been developed and are available for use in clinical practice.
Methods: The performance of Parasight All-in-One (AIO), a second-generation device, was evaluated by comparing it to a well-accepted research method (mini-FLOTAC) and to another commercially available test (Imagyst). Fifty-nine canine and feline infected fecal specimens were quantitatively analyzed by all three methods.
Relative egg extraction efficiencies of manual and automated fecal egg count methods in equines.
Helminthologia
March 2024
Parasight System Inc., Suite 2130, 1532 N. Limestone St., Lexington, KY 40505, USA.
The World Association for the Advancement of Veterinary Parasitology recently released new recommendations for the design of fecal egg count (FEC) reduction tests for livestock. These provide suggestions as to the number of animals to be sampled and the minimum number of eggs that must be counted to produce statistically meaningful results. One of the considerations for study design is the multiplication factor of the FEC method to be used; methods with lower multiplication factors require fewer animals to be sampled because they are presumed to count more eggs per test.
View Article and Find Full Text PDFDevelopment and performance of an automated fecal egg count system for small ruminant strongylids.
Vet Parasitol
July 2021
M.H. Gluck Equine Research Center, Department of Veterinary Science, University of Kentucky, 1400 Nicholasville Road, Lexington, KY, 40546, USA. Electronic address:
An automated equine fecal egg count test, known as the Parasight System, was modified for use with small ruminants. Modifications included the introduction of a short centrifugation step in a floatation medium, an adjustment in pre-test sample filtering, and training of an image analysis-based egg counting algorithm to recognize and enumerate trichostrongylid eggs. In preliminary assessments, the modified method produced trichostrongylid egg counts comparable to manual McMaster analyses of the same samples from both ovine and caprine sources.
View Article and Find Full Text PDFComparision of the various routine diagnostic modalities of malaria and a new method: the Parasight™ platform.
J Parasit Dis
September 2020
Department of Microbiology, Jawaharlal Institute of Postgraduate Medical Education and Research, 2nd Floor, institute Block, JIPMER, Dhanvantri Nagar, Puducherry, 605006 India.
Malaria is one of most important parasitic disease, which is still much prevalent in India. The burden of malaria in India is complex and the proportions of and vary across India, because of the highly variable malaria eco-epidemiological profiles, transmission factors, and the presence of multiple species and vectors. The diagnostic modalities which were being used currently, are at the risk of missing potential malaria cases, if a single test is being used for a given sample.
View Article and Find Full Text PDF[Obstacles to laboratory diagnosis of malaria in Mali--perspectives].
Med Trop (Mars)
April 2010
Département d'Epidémiologie des Affections Parasitaires (DEAP)/Faculté de Médecine, de Pharmacie et d'Odonto-Stomatologie, Bamako, Mali.
Rapid accurate laboratory diagnosis is necessary for effective malaria management. In Mali, meeting this prerequisite is impeded by underuse of laboratory diagnosis by clinicians, absence of qualified laboratory facilities in some locations, and poor continuous professional education of laboratory technicians. The twofold aim of this investigation was to perform quality control of thick smear readings made by laboratory technicians in 1998 and 1999 and to study the feasibility and diagnostic value of two rapid diagnostic tests (RDT), i.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!