AI Article Synopsis
- The study aimed to characterize a recombinant antigen of Ancylostoma caninum through immunoscreening with specific antisera.
- The in vitro expression of the clone produced a protein around 34 kDa, which was identified in Western blots but not recognized by sera from infected dogs or rabbits.
- DNA analysis showed significant similarity to the paramyosin gene from C. elegans, suggesting a strong homology and indicating its potential role in muscle tissue recognition.
Article Abstract
The objective of this study was to characterise a recombinant antigen of Ancylostoma caninum that had been identified by immunoscreening with selected antisera described elsewhere. In vitro expression of clone 341 produced a protein with an apparent molecular mass of approximately 34 kDa which was recognised in Western blots by antisera against whole worms and antisera against esophagi from adult worms, but not by sera from experimentally infected dogs or rabbits. DNA sequencing showed a cDNA of 1176 bp coding for a 34-kDa protein, similar to the size identified in the immunoblot. DNA database comparison revealed an 80-82% homology with the Caenorhabditis elegans unc-15 gene coding for paramyosin. The deduced aa sequence of clone 341 showed 95% homology with the paramyosin aa sequence of C. elegans. Affinity purified antibodies against the recombinant protein recognise a protein with an apparent molecular mass of 97 kDa of A. caninum muscle tissue fraction which is in accordance with the molecular mass of paramyosin from Schistosoma mansoni and Schistosoma japonicum.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/s0020-7519(98)00106-4 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Cu Site-Enhanced Adsorption and Crown Ether-Reconfigured Interfacial DO Promote Electrocatalytic Dehalogenative Deuteration.
J Am Chem Soc
January 2025
Department of Chemistry, School of Science, Tianjin University, Tianjin 300072, China.
Electrocatalytic dehalogenative deuteration is a sustainable method for precise deuteration, whereas its Faradaic efficiency (FE) is limited by a high overpotential and severe D evolution reaction (DER). Here, Cu site-adjusted adsorption and crown ether-reconfigured interfacial DO are reported to cooperatively increase the FE of dehalogenative deuteration up to 84% at -100 mA cm. Cu sites strengthen the adsorption of aryl iodides, promoting interfacial mass transfer and thus accelerating the kinetics toward dehalogenative deuteration.
View Article and Find Full Text PDFFirst report of strawberry root rot caused by in China.
Plant Dis
January 2025
Hebei Academy of Agricultural and Forestry Sciences, Plant Protection Institute, 437 Dongguan Street, Baoding, Hebei, China, 071000.
Strawberry () is an important economic crop in Hebei, China. In May 2023, root rot was observed in strawberry plantations (cultivar 'Benihoppe') in Shijiazhuang (37°57'23″N, 115°16'34″E), Hebei, China. The incidence of the disease reached up to 30% in the field.
View Article and Find Full Text PDFA Comprehensive Evaluation of the Neuropharmacological Potential of Methanolic Leaf Extract of Acanthus ebracteatus (Vahl.) Using Experimental and In Silico Approaches.
Chem Biodivers
January 2025
Department of Pharmaceutical Sciences, College of Health Sciences and Pharmacy, Chicago State University, Chicago, Illinois, USA.
This study was undertaken to assess the antioxidant and neuropharmacological potentials of the methanol leaf extract of Acanthus ebracteatus (MAEL) through experimental and in silico methods. The phytochemical screening (PS) and GC-MS (gas chromatography-mass spectrometry) identified 28 phytochemicals with different classes in nature in MAEL. The MAEL revealed better antioxidant activity through various in vitro antioxidant assays.
View Article and Find Full Text PDFAdipose tissue transcriptome in patients switching efavirenz or a protease inhibitor to raltegravir compared to people without HIV.
AIDS
January 2025
Obesity Research Unit, Research Program for Clinical and Molecular Metabolism, Faculty of Medicine, University of Helsinki, Helsinki, Finland.
Objective: To study the subcutaneous adipose tissue (SAT) transcriptome in people with HIV (PWH) switching efavirenz (EFV) or a protease inhibitor (PI) to raltegravir and to compare the transcriptome of PWH to those of people without HIV (PWoH).
Design: PWH (n = 36) on EFV (n = 22) or a PI (n = 14) based ART regimen were randomized to switch to RAL (n = 15) or to continue unchanged medication (n = 17). PWoH (n = 10), comparable in age and body mass index, were included for comparison.
Integrin Trafficking, Fibronectin Architecture, and Glomerular Injury upon AdipoR1 Depletion.
J Am Soc Nephrol
January 2025
Research Program for Clinical and Molecular Metabolism, Faculty of Medicine, University of Helsinki, Helsinki, Finland.
Background: Deficiency of adiponectin and its downstream signaling may contribute to the pathogenesis of kidney injury in type 2 diabetes. Adiponectin activates intracellular signaling via adiponectin receptors 1 and 2 (AdipoR1 and AdipoR2), but the role of AdipoR-mediated signaling in glomerular injury in type 2 diabetes remains unknown.
Methods: The expression of AdipoR1 in the kidneys of people with type 2 diabetes and the expression of podocyte proteins or injury markers in the kidneys of AdipoR1-knockout (AdipoR1-KO) mice and immortalized AdipoR1-deficient human podocytes were investigated by immunohistochemistry and immunoblotting.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!