Crystals of chloroplast NADP-dependent malate dehydrogenase have been grown both with and without the cofactor NADP present. The enzyme has a molecular weight of 43 kDa per subunit and exists as a dimer in solution. The crystals diffract to 2.8 A and belong to the space group P3221 with cell dimensions a = 148.1, c = 65.5 A.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1107/s0907444997015655 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Single-cell RNA-sequencing provides new insights into the cell-specific expression patterns and transcriptional regulation of photosynthetic genes in bermudagrass leaf blades.
Plant Physiol Biochem
August 2024
Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, China.
As an important warm-season turfgrass species, bermudagrass (Cynodon dactylon L.) flourishes in warm areas around the world due to the existence of the C4 photosynthetic pathway. However, how C4 photosynthesis operates in bermudagrass leaves is still poorly understood.
View Article and Find Full Text PDFA Semi-throughput Procedure for Assaying Plant NADP-malate Dehydrogenase Activity Using a Plate Reader.
Bio Protoc
August 2023
Université Paris-Saclay, CNRS, INRAE, Université Evry, Institute of Plant Sciences Paris-Saclay (IPS2), Gif sur Yvette, France.
Chloroplast NADP-dependent malate dehydrogenase (NADP-MDH) is a redox regulated enzyme playing an important role in plant redox homeostasis. Leaf NADP-MDH activation level is considered a proxy for the chloroplast redox status. NADP-MDH enzyme activity is commonly assayed spectrophotometrically by following oxaloacetate-dependent NADPH oxidation at 340 nm.
View Article and Find Full Text PDFChange in expression levels of NAD kinase-encoding genes in Flaveria species.
J Plant Physiol
October 2021
Graduate School of Science and Engineering, Saitama University, 225 Shimo-Okubo, Sakura-ku, Saitama-city, Saitama 338-8570, Japan. Electronic address:
Nicotinamide adenine dinucleotides (NAD(H)) and NAD phosphates (NADP(H)) are electron carriers involved in redox reactions and metabolic processes in all organisms. NAD kinase (NADK) is the only enzyme that phosphorylates NAD into NADP, using ATP as a phosphate donor. In NADP-dependent malic enzyme (NADP-ME)-type C photosynthesis, NADP(H) are required for dehydrogenation by NADP-dependent malate dehydrogenase (NADP-MDH) in mesophyll cells, and decarboxylation by NADP-ME in bundle sheath cells.
View Article and Find Full Text PDFUpregulation of bundle sheath electron transport capacity under limiting light in C Setaria viridis.
Plant J
June 2021
Australian Research Council Centre of Excellence for Translational Photosynthesis, Division of Plant Science, Research School of Biology, The Australian National University, Acton, Australian Capital Territory, 2601, Australia.
C photosynthesis is a biochemical pathway that operates across mesophyll and bundle sheath (BS) cells to increase CO concentration at the site of CO fixation. C plants benefit from high irradiance but their efficiency decreases under shade, causing a loss of productivity in crop canopies. We investigated shade acclimation responses of Setaria viridis, a model monocot of NADP-dependent malic enzyme subtype, focussing on cell-specific electron transport capacity.
View Article and Find Full Text PDFAntagonistic Regulation by CPN60A and CLPC1 of TRXL1 that Regulates MDH Activity Leading to Plant Disease Resistance and Thermotolerance.
Cell Rep
December 2020
Noble Research Institute, Ardmore, OK 73401, USA. Electronic address:
Global warming and emerging plant diseases challenge agricultural food/feed production. We identify mechanism(s) regulating both plant thermotolerance and disease resistance. Using virus-induced gene silencing (VIGS)-based genetic screening, we identify a thioredoxin-like 1 (TRXL1) gene involved in plant nonhost disease resistance and thermotolerance.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!