Studies of the guinea pig small intestine have suggested that serotonin (5-HT) may be a mucosal transmitter that stimulates sensory nerves and initiates peristaltic and secretory reflexes. We tested the hypothesis that guinea pig villus epithelial cells are able to inactivate 5-HT because they express the same 5-HT transporter as serotonergic neurons. A full-length cDNA, encoding a 630-amino acid protein (89.2% and 90% identical, respectively, to the rat and human 5-HT transporters) was cloned from the guinea pig intestinal mucosa. Evidence demonstrating that this cDNA encodes the guinea pig 5-HT transporter included 1) hybridization with a single species of mRNA ( approximately 3.7 kb) in Northern blots of the guinea pig brain stem and mucosa and 2) uptake of [3H]5-HT by transfected HeLa cells via a saturable, high-affinity (Michaelis constant 618 nM, maximum velocity 2.4 x 10(-17) mol . cell-1 . min-1), Na+-dependent mechanism that was inhibited by chlorimipramine > imipramine > fluoxetine > desipramine > zimelidine. Expression of the 5-HT transporter in guinea pig raphe and enteric neurons and the epithelium of the entire crypt-villus axis was demonstrated by in situ hybridization and immunocytochemistry. Inhibition of mucosal 5-HT uptake potentiates responses of submucosal neurons to mucosal stimulation. The epithelial reuptake of 5-HT thus appears to be responsible for terminating mucosal actions of 5-HT.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1152/ajpgi.1998.275.3.G433 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Establishment of a Mouse Model with Cough Hypersensitivity via Inhalation of Citric Acid.
J Vis Exp
January 2025
State Key Laboratory of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University;
Cough is one of the most common symptoms of many respiratory diseases. Chronic cough significantly impacts quality of life and imposes a considerable economic burden. Increased cough sensitivity is a pathophysiological hallmark of chronic cough.
View Article and Find Full Text PDFThe Role of Thickening Agent Proportions in Optimizing Nanoemulsion Gel for Dermatophytosis Treatment.
Int J Nanomedicine
January 2025
College of Animal Science, Guizhou University, Guiyang, Guizhou, People's Republic of China.
Background: Adjusting thickening agent proportions in nanoemulsion gel (NG) balances its transdermal and topical delivery properties, making it more effective for dermatophytosis treatment.
Methods: Carbomer 940 and α-pinene were used as model thickening agent and antifungal, respectively. A series of α-pinene NGs (αNG1, αNG2, αNG3) containing 0.
Real-Time 5-Hydroxyindoleacetic Acid Monitoring in Guinea Pig Brain Using a Molecular Imprinted Polymer-Based Galvanic Redox Potentiometric Sensor.
Anal Chem
January 2025
College of Chemistry, Beijing Normal University, Beijing 100875, China.
5-Hydroxyindoleacetic acid (5-HIAA), a vital metabolite of serotonin (5-HT), is crucial for understanding metabolic pathways and is implicated in various mental disorders. In situ monitoring of 5-HIAA is challenging due to the lack of affinity ligands and issues with electrochemical fouling. We present an advanced sensing approach that integrates customizable molecular imprinting polymer (MIP) with self-driven galvanic redox potentiometry (GRP) for precise, real-time in vivo monitoring of 5-HIAA.
View Article and Find Full Text PDFPerception and utilisation of veterinary services by rodent owners in the United Kingdom.
Vet Rec
January 2025
Department of Animal and Agriculture, Hartpury University, Gloucester, UK.
Background: There is limited research on how rodent owners use and perceive veterinary services and what the demand for pet insurance for these species is.
Methods: An online survey of owners of pet rodents (guinea pigs, hamsters, rats, gerbils and mice) measured owner confidence in recognising signs of illness, their opinions on and use of veterinary services and their willingness to purchase pet insurance.
Results: A total of 1700 respondents completed the survey.
Novel Hsp90α inhibitor inhibits HSV-1 infection by suppressing the Akt/β-catenin pathway.
Int J Antimicrob Agents
January 2025
School of Pharmacy, Shenzhen University Medical School, Shenzhen University, Shenzhen 518055, China. Electronic address:
The prevalence of herpes simplex virus type 1 (HSV-1) infection and the emergence of drug-resistant HSV-1 strains posts a significant global health challenge, necessitating the urgent development of effective anti-HSV-1 drugs. As one of the most prevalent molecular chaperones, heat shock protein 90 α (Hsp90α) has been extensively demonstrated to regulate a range of viral infections, thus representing a promising antiviral target. In this study, we identified JD-13 as a novel Hsp90α inhibitor and explored its capability in inhibiting HSV-1 infection.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!