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Heterotypic spheroids as a strategy for 3D culture of cryopreserved primary human hepatocytes in stirred-tank systems.
SLAS Discov
January 2025
iBET, Instituto de Biologia Experimental e Tecnológica, Apartado 12 2781-901 Oeiras, Portugal; ITQB NOVA, Instituto de Tecnologia Química e Biológica António Xavier, Av. Republica, 2780-157, Oeiras, Portugal. Electronic address:
Primary human hepatocytes (PHHs) are the preferred cell source to address liver function. Despite originating from the native tissue, one of the bottlenecks when using primary material is the donor-to-donor variability. Cryopreserved PHHs offer a high number of cells from the same donor and standardization of cell isolation and cryopreservation procedures, mitigating some of the inter-donor variability.
View Article and Find Full Text PDFComparison of obstetrical and neonatal outcomes between fresh versus frozen-thawed testicular sperm derived from microTESE.
J Assist Reprod Genet
October 2024
IVF and Infertility Unit, Department of Obstetrics and Gynecology, Shaare Zedek Medical Center, affiliated with the Faculty of Medicine, Hebrew University of Jerusalem, Jerusalem, Israel.
Article Synopsis
- The study aims to compare outcomes of embryo transfer cycles using either fresh or frozen-thawed testicular sperm from non-obstructive azoospermia (NOA) patients.
- A retrospective analysis of 48 couples involved 93 embryo transfer cycles, with similar rates of pregnancy outcomes and no significant clinical differences in birth weights or complications between both sperm groups.
- The results indicate that neither fresh nor frozen-thawed sperm significantly affects obstetrical outcomes or neonatal birth weight, suggesting both can be used effectively.
Ultrastructural examination of cryodamage in Paracentrotus lividus eggs during cryopreservation.
Sci Rep
April 2024
Centro de Investigación Mariña (CIM), Departamento de Ecoloxía e Bioloxía Animal, Grupo ECOCOST, Universidade de Vigo, 36208, Pontevedra, Spain.
This study examinates the challenges of cryopreserving sea urchin (Paracentrotus lividus) eggs, a task hindered by factors like low membrane permeability and high sensitivity to cryoprotective agents (CPAs). While successful cryopreservation has been achieved for some marine invertebrates, eggs remain problematic due to their unique characteristics. The study explores the impact of various CPAs and cryopreservation techniques on sea urchin eggs, employing scanning and transmission electron microscopy to analyze cellular damage.
View Article and Find Full Text PDFCombinations of arginine and pullulan reveal the selective effect of stabilization mechanisms on different lyophilized proteins.
Int J Pharm
April 2024
Department of Pharmaceutical Technology and Biopharmacy, University of Groningen, Antonius Deusinglaan 1, 9713 AV Groningen, the Netherlands. Electronic address:
The stability of lactate dehydrogenase (LDH) and β-galactosidase (β-gal), incorporated in arginine/pullulan (A/P) mixtures at various weight ratios by lyophilization, was determined. The physicochemical characteristics of various A/P mixtures were assessed. With decreasing A/P ratios, the glass transition temperature of the formulations increased.
View Article and Find Full Text PDFOverexpression of VEGFα as a biomarker of endothelial dysfunction in aortic tissue of α-GAL-Tg/KO mice and its upregulation in the serum of patients with Fabry's disease.
Front Cardiovasc Med
February 2024
Department of Cardiology, University Heart & Vascular Center Hamburg, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
Introduction: Fabry's disease is an X-linked lysosomal storage disorder caused by reduced activity of α-galactosidase A (GAL), leading to premature death on account of renal, cardiac, and vascular organ failure. Accumulation of the GAL substrate globotriaosylceramide (Gb3) in endothelial and smooth muscle cells is associated with early vascular cell damage, suggesting endothelial dysfunction as a driver of cardiorenal organ failure. Here, we studied the vascular expression of the key angiogenic factors, VEGFα and its antagonist angiostatin, in Fabry α-GAL-Tg/KO mice and determined circulating VEGFα and angiostatin serum levels in patients with Fabry's disease and healthy controls.
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