Slaframine was derivatized precolumn with fluorescamine. The derivatized slaframine was chromatographed isocratically using HPLC on a Hamilton PRP-1 C18 polymeric column with fluorescence detection. By using fluorescent derivatization, sensitivity was increased 100-fold over previously reported GC methods. A liquid-liquid partition was used to extract slaframine from plasma with a 95% recovery and a CV% of 8.4. A solid-phase extraction was used to extract slaframine from milk with a 91% recovery and a CV% of 9.8.
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