AI Article Synopsis
- Researchers have sequenced a multi-gene family of cuticular proteins from the mosquito Anopheles gambiae, identifying three main genes (agcp2a-c) within a larger genomic clone.
- Analysis of cDNAs suggests the presence of at least four related genes, with a high sequence identity of 95%, but some divergence noted in the 3' ends and repetitive sequences.
- In situ hybridization studies map these genes to specific chromosome loci and indicate that transcription occurs primarily in the pupal stage, with proteins showing similarities to other known cuticular proteins in other species.
Article Abstract
We have cloned and sequenced members of a cuticular protein multi-gene family from the mosquito Anopheles gambiae. Three genes (agcp2a-c), each approximately 1 kb in length, were found in a 17.4 kb genomic phage clone. Analysis of ten cDNAs revealed that at least four related genes are present. The open reading frame of the genes and cDNAs showed 95% sequence identity. Divergence was observed in the sequence of the 3' ends and the number of copies of two repeated coding sequences. In situ hybridizations with a probe prepared from one of these circular protein genes physically mapped to two loci, 26B on chromosome 2L and 37A on 3R. Transcription of these An. gambiae cuticular protein genes appears to be limited to pharate pupae and the expressed protein(s) is found in early pupae. The deduced amino acid sequence of these proteins contains a hydrophilic region with significant similarity to other cuticular proteins including the pupal-specific cuticular protein, EDG84, of Drosophila melanogaster (Apple and Fristrom).
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| http://dx.doi.org/10.1016/s0965-1748(98)00016-2 | DOI Listing |
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