AI Article Synopsis
- Two Lactobacillus isolates, Lact. acidophilus I 26 and Lact. fermentum I 25, were chosen for their weak aggregation with E. coli and strong adhesion to ileal epithelial cells (IEC).
- The study examined these isolates' interactions with different E. coli strains using three approaches: exclusion, competition, and displacement.
- Results showed that neither Lactobacillus isolate could significantly prevent E. coli from adhering to IEC, except for a slight reduction in attachment of E. coli O1:K1 by Lact. fermentum I 25 during the competition test.
Article Abstract
Two Lactobacillus isolates, Lact. acidophilus I 26 and Lact. fermentum I 25, were selected, based on their poor aggregation with Escherichia coli and strong ability to adhere to ileal epithelial cells (IEC), to study in vitro interactions with E. coli O1:K1, O2:K1 and O78:K80 in an IEC radioactive-assay under the conditions of exclusion (lactobacilli and IEC, followed by the addition of E. coli), competition (lactobacilli, IEC and E. coli together) and displacement (E. coli and IEC, followed by the addition of lactobacilli). The results indicated that Lact. acidophilus I 26 and Lact. fermentum I 25 could not significantly reduce the attachment of E. coli O1:K1, O2:K1 and O78:K80 to IEC under the three conditions tested in vitro, except that the attachment of E. coli O1:K1 was slightly reduced by Lact. fermentum I 25 in the test for competition.
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| http://dx.doi.org/10.1046/j.1365-2672.1998.00434.x | DOI Listing |
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