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Choosing the decolourizer and its strength to stain Mycobacterium leprae. Does it actually matter? | LitMetric

Choosing the decolourizer and its strength to stain Mycobacterium leprae. Does it actually matter?

Lepr Rev

NSL-South Sulawesi Leprosy Control Project, Provincial Health Services, South Sulawesi, Indonesia.

Published: June 1998

AI Article Synopsis

  • Leprosy bacilli are easier to decolourize during staining compared to tuberculosis bacilli, requiring a weaker decolourizer concentration.
  • In Indonesia, a 'strong' decolourizer is used for both bacteria, which may not be optimal.
  • A study found no significant difference in bacterial index results when using various concentrations of decolourizers, indicating that the same staining technique might work for both tuberculosis and leprosy.

Article Abstract

Leprosy bacilli are more easily decolourized during staining than tuberculosis bacilli, so a weaker concentration of decolourizer is usually recommended. In Indonesia, the same 'strong' decolourizer is used for identifying both organisms. In a study to compare the results using different concentrations of different decolourizers, no difference could be found in the bacterial index (BI). It is suggested that the same staining technique can be used for tuberculosis and leprosy.

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Source
http://dx.doi.org/10.5935/0305-7518.19980013DOI Listing

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