Anti-nucleosome antibodies, which recognise conformational epitopes consisting of histone and DNA in chromatin, have been described in autoimmune diseases. In this study, an attempt was made to isolate anti-nucleosome antibodies from the anti-DNA-depleted plasma IgG of two lupus patients either with or without nephritis by nucleohistone affinity chromatography. The purified nucleohistone-binding antibodies bound to nucleohistone in a specific manner and contained enriched anti-histone antibodies. However, adsorption of the purified antibodies with histone revealed that the nephritis patient-derived antibodies contained nucleohistone-specific antibodies. Although such purified antibodies may not recognise native structures of nucleosomes, this chromatography may provide a method to isolate and determine the fine specificity of anti-nucleosome antibodies in various autoimmune diseases.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/BF01451045 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
An Unusual Case of Overlapping Immunoglobulin G4-Related Disease and Systemic Lupus Erythematosus.
Cureus
December 2024
Nephrology, Unidade Local de Saúde de São José, Lisbon, PRT.
Immunoglobulin G4-related disease (IgG4-RD) and systemic lupus erythematosus (SLE) are multisystemic autoimmune disorders that can present with renal manifestations. Overlapping cases of these diseases are extremely rare and present both diagnostic and therapeutic challenges. We report the case of a 70-year-old male with a history of autoimmune pancreatitis, who was admitted with fatigue, weight loss, and worsening kidney function.
View Article and Find Full Text PDFIntegrating clinical data and genetic susceptibility to elucidate the relationship between systemic lupus erythematosus and human cytomegalovirus infection.
Virol J
November 2024
Department of Rheumatology and Clinical Immunology, Peking Union Medical College Hospital, Key Laboratory of Rheumatology & Clinical Immunology, Chinese Academy of Medical Sciences & Peking Union Medical College, Ministry of Education, National Clinical Research Center for Dermatologic and Immunologic Diseases (NCRC-DID), Beijing, China.
Background: Viral infections are known to induce the occurrence and pathogenesis of systemic lupus erythematosus (SLE). Previous studies have indicated a possible relationship between SLE and human cytomegalovirus (HCMV) infection and have attributed HCMV to be associated with various autoantibodies; however, these studies were constrained by variations in sample size and potential selection bias. Therefore, in the present study, we aimed to elucidate the relationship between HCMV and autoantibodies in patients with SLE by integrating clinical data and genetic susceptibility.
View Article and Find Full Text PDFClinical Profile and Outcomes of Patients With Systemic Lupus Erythematosus.
Cureus
September 2024
Internal Medicine, Byramjee Jeejeebhoy Government Medical College and Hospital, Pune, IND.
Article Synopsis
- Systemic lupus erythematosus (SLE) is a complex autoimmune disease impacting various organs, and this study aims to enhance early detection and management through comprehensive clinical profiling at diagnosis.
- Conducted in Pune, India, the study involved 80 patients diagnosed with SLE, using detailed clinical assessments and follow-ups to track disease activity over 1.5 years.
- Results showed that the majority of the patients were young women, with a variety of symptoms, including a high prevalence of oral ulcers, indicating the diverse clinical manifestations of SLE.
Comparison of Clinical and Laboratory Characteristics in Lupus Nephritis vs. Non-Lupus Nephritis Patients-A Comprehensive Retrospective Analysis Based on 921 Patients.
J Clin Med
July 2024
Jagiellonian University Medical College, Department of Rheumatology and Immunology, Jakubowskiego 2, 30-688 Kraków, Poland.
Article Synopsis
- Lupus nephritis (LN) is a kidney inflammation linked to systemic lupus erythematosus (SLE), and the study investigated the clinical and laboratory differences between LN and non-LN SLE patients at a university hospital in Kraków, Poland.
- Out of 921 SLE patients analyzed, 331 had LN, which presented at a younger age (29 vs. 37 years) and a higher male proportion compared to non-LN patients, who also had a higher occurrence of certain antibodies like anti-cardiolipin IgM.
- LN patients showed more severe symptoms, required more immunosuppressive treatment, and had specific risk factors for experiencing multiple LN flares, underscoring the need for
Critical peripheral ischemia: Higher prevalence in Indian systemic lupus erythematosus inception cohort for research (INSPIRE).
Lupus
August 2024
Department of Clinical Immunology and Rheumatology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow, India.
Article Synopsis
- The study examines digital ischemia, specifically digital infarcts and gangrene, in patients with systemic lupus erythematosus (SLE) within the Indian SLE inception cohort (INSPIRE).
- Out of 2503 patients, 75 (2.9%) exhibited critical peripheral ischemia (CPI), predominantly affecting women, with many cases leading to amputations.
- Key associations with CPI include pulmonary hypertension and antiphospholipid antibody positivity, while specific types of lupus nephritis appear to reduce risk; the short-term mortality rates were similar between those with CPI and controls.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!