AI Article Synopsis
- High-density oligonucleotide arrays (DNA chips) were used for two-color mutational analysis of the BRCA1 gene, focusing on a specific exon related to hereditary breast and ovarian cancer.
- The two-color analysis method allows for competitive hybridization, enhancing the assay's performance by comparing an unknown sample against a reference standard.
- This study introduces a new dye combination (phycoerythrin and phycoerythrin*cy5) that improves signal intensity balance and reduces spectral overlap while still being compatible with a 488 nm excitation source.
Article Abstract
High density oligonucleotide arrays (DNA chips) have been used in two color mutational analysis of the 3.43 kb exon 11 of the hereditary breast and ovarian cancer gene BRCA1 . Two color analysis allows competitive hybridization between a reference standard and an unknown sample, improving the performance of the assay. Fluorescein and phycoerythrin dyes werepreviously used due to their compatibility with a single line 488 nm excitation source. Here we show that an alternative dye combination, containing the energy transfer dye system phycoerythrin*cy5 along with phycoerythrin, provides more evenly matched signal intensities and decreased spectral overlap between the two fluorophores, while maintaining compatibility with a 488 nm excitation source.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC147769 | PMC |
| http://dx.doi.org/10.1093/nar/26.16.3865 | DOI Listing |
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