Fifty-five novel rat microsatellite markers were isolated from libraries specific for rat chromosomes (Chrs) 1, 2, and 7. The markers were mapped in three backcross rat populations. Thirty of these markers mapped to Chrs 1, 2, or 7, while the other 25 mapped to other chromosomes. New markers for two genes, liver-specific transporter gene (Livtr) and insulin-responsive glucose transporter (Glut4), were also mapped to rat Chrs 9 and 10, respectively. Three provisionally assigned markers from previous studies were also confirmed. Detailed methodologies for the generation and enrichment of clones containing repeat sequences and for the isolation of chromosome-specific markers are presented, since they represent unique combinations and modifications of previous protocols. Such methods and the newly presented markers should be useful for both specific and general mapping studies in the rat.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s003359900833 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Genetic Characteristics of the Rat Fibroblast Cell Line Rat-1.
Cells
December 2024
Institute of Molecular Pathobiochemistry, Experimental Gene Therapy and Clinical Chemistry (IFMPEGKC), RWTH, University Hospital Aachen, D-52074 Aachen, Germany.
The Rat-1 cell line was established as a subclone of the parental rat fibroblastoid line F2408, derived from Fisher 344 rat embryos. Rat-1 cells are widely used in various research fields, especially in cancer biology, to study the effects of oncogenes on cell proliferation. They are also crucial for investigating signal transduction pathways and play a key role in drug testing and pharmacological studies due to their rapid proliferation.
View Article and Find Full Text PDFExploring the molecular profile of localized colon cancer: insights from the AIO Colopredict Plus registry.
Front Oncol
November 2024
Institute of Pathology, Ruhr University, Bochum, Germany.
Introduction: Understanding the mutational landscape of colon cancer (CC) is crucial for targeted therapy development. Microsatellite instability (MSI-H), rat sarcoma (RAS), and B-Raf proto-oncogene, serine/threonine kinase (BRAF) mutations (MT) are pivotal markers. Further investigation into clinicopathological features of RAS and BRAF MT in microsatellite stable (MSS) and MSI-H tumors is warranted.
View Article and Find Full Text PDFAmerican College of Surgeons Cancer Program Annual Report from 2021 Participant User File.
J Am Coll Surg
January 2025
Surgery (Boughey), Mayo Clinic Rochester, MN.
The National Cancer Database (NCDB) collects data from approximately 1,500 Commission on Cancer (CoC) facilities and represent 73.7% of newly diagnosed cancers nationwide. The American College of Surgeons Cancer Program developed it first annual report from the NCDB 2021 participant user file reporting new observations and recent trends of cancer diagnoses, patient demographics, and treatments as well as an in-depth report on treatment and outcomes in breast, pancreas, and colon cancers.
View Article and Find Full Text PDFAltitude shapes gut microbiome composition accounting for diet, thyroid hormone levels, and host genetics in a subterranean blind mole rat.
Front Microbiol
November 2024
Department of Biology, Faculty of Science, Bülent Ecevit University, Zonguldak, Türkiye.
Article Synopsis
- - The study examined how altitude affects the gut microbiome of Anatolian Blind Mole Rats using 16S rRNA metabarcoding, noting significant changes in bacterial composition based on elevation, particularly with facultatively anaerobic bacteria being more abundant at higher altitudes.
- - Microbiome alpha diversity was highest at mid-altitude, showing a mix of low and high elevation elements, while beta diversity was notably influenced by altitude; however, diet composition varied with altitude without affecting its diversity.
- - There was no clear genetic structure related to altitude among the host populations, and while free thyroxine levels increased with altitude, no specific bacteria correlated with hormone levels, although total thyroxine showed a positive connection to microbiome diversity.
Association of Mutation and Gene Pathways in Colorectal Carcinoma: A Transcriptome- and Methylome-Wide Study and Potential Implications for Therapy.
Int J Mol Sci
July 2024
Institute for Population and Precision Health, Biological Sciences Division, The University of Chicago, Chicago, IL 60637, USA.
Kirsten Rat Sarcoma () is the most commonly mutated oncogene in colorectal carcinoma (CRC). We have previously reported the interactions between microsatellite instability (MSI), DNA promoter methylation, and gene expression. In this study, we looked for associations between mutation, gene expression, and methylation that may help with precision medicine.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!