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Portable pH meter-based competitive immunoassay of E-selectin using urease-encapsulated metal-organic frameworks.
Talanta
January 2025
Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education of China), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha, 410081, PR China. Electronic address:
E-selectin (CD62E) is an adhesion molecule expressed on the surface of endothelial cells (ECs) and its level increases significantly upon the stimulation of ECs by inflammatory factors. Quantitative analysis of CD62E is of great importance to early diagnosis and treatment of vascular diseases and hypertension. A new method for the determination of CD62E was developed using a portable pH meter in this work.
View Article and Find Full Text PDFDevelopment of a Luciferase Immunosorbent Assay for Detecting Crimean-Congo Hemorrhagic Fever Virus IgG Antibodies Based on Nucleoprotein.
Viruses
December 2024
Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, Guangzhou 510515, China.
Crimean-Congo hemorrhagic fever (CCHF) is a serious tick-borne disease with a wide geographical distribution. Classified as a level 4 biosecurity risk pathogen, CCHF can be transmitted cross-species due to its aerosol infectivity and ability to cause severe hemorrhagic fever outbreaks with high morbidity and mortality. However, current methods for detecting anti-CCHFV antibodies are limited.
View Article and Find Full Text PDFNeuroprotective Actions of Cannabinoids in the Bovine Isolated Retina: Role of Hydrogen Sulfide.
Pharmaceuticals (Basel)
January 2025
Department of Pharmaceutical Sciences, College of Pharmacy and Health Sciences, Texas Southern University, Houston, TX 77004, USA.
Both hydrogen sulfide and endocannabinoids can protect the neural retina from toxic insults under in vitro and in vivo conditions. The aim of the present study was two-fold: (a) to examine the neuroprotective action of cannabinoids [methanandamide and 2-arachidonyl glycerol (2-AG)] against hydrogen peroxide (HO)-induced oxidative damage in the isolated bovine retina and (b) to evaluate the role of endogenously biosynthesized hydrogen sulfide (HS) in the inhibitory actions of cannabinoids on the oxidative stress in the bovine retina. Isolated neural retinas from cows were exposed to oxidative damage using HO (100 µM) for 10 min.
View Article and Find Full Text PDFSeroprevalence of , , , , , , and in Human Population from Eastern Poland.
Pathogens
January 2025
Department of Health Biohazards and Parasitology, Institute of Rural Health, Jaczewskiego 2, 20-090 Lublin, Poland.
The epidemiological situation related to infectious diseases is influenced by many factors. To monitor actual trends in selected zoonoses, a total of 473 serum samples from farmers, forestry workers, and veterinarians were collected for serological examination. Anti- sensu lato (s.
View Article and Find Full Text PDFSerological Diagnosis of Infection in Cetaceans by Rapid Serum Agglutination Test and Competitive ELISA with and as Antigens.
Pathogens
January 2025
Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise 'G. Caporale', National Reference Center for Brucellosis, 64100 Teramo, Italy.
Rose Bengal antigen and smooth lipopolysaccharide (s-LPS) were produced from a field strain of ("homologous" antigens) and from the reference strain S99 ("heterologous" antigens); they are currently used for the diagnosis of brucellosis in cattle, water buffaloes, sheep, goats, and pigs, as recommended in the Manual of Diagnostic Tests and Vaccines for Terrestrial Animals of the World Organization for Animal Health (WOAH). "Homologous" and "heterologous" antigens were used in a rapid serum agglutination test (Rose Bengal test, RBT) and a competitive ELISA assay (c-ELISA) to test a panel of sera, blood, and other body fluids (cerebrospinal fluid, pericardial fluid, tracheal fluid, and aqueous humor) collected from 71 individuals belonging to five cetacean species (; ; ; ; and ), which were found stranded on the Italian coastline. Six animals were positive for spp.
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