A total of 51 strains of non-typable Haemophilus influenzae isolated from a single hospital were subjected to analysis of biochemical traits, outer membrane protein (OMP) profiles, lipopolysaccharide (LPS) electrophoresis and antibiotic susceptibility determination in order to establish a method for bacterial typing. Results proved that none of the methods tested were useful for typing purposes, although the data showed that (1) OMP profiles were extremely variable; (2) LPS profiles were uniform; (3) the multiresistant strains manifested different resistance patterns; and (4) biochemically, all strains were closely related.
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Microorganisms
December 2024
Laboratory of Microbiology, Children's Hospital of Tunis, Beb Saadoun, Tunis 1007, Tunisia.
The changing epidemiological profile of invasive infections (IIHi) is noted in the post-vaccination era. The aim of this study was to characterize phenotypically and genotypically invasive (Hi) isolates detected in Tunisian pediatric patients. A retrospective study was conducted in the microbiology laboratory of the Children's Hospital of Tunis over ten years (2013-2023).
View Article and Find Full Text PDFJ Epidemiol Glob Health
June 2024
Thai Binh University of Medicine and Pharmacy, Thai Binh, Vietnam.
Among 467 children under five hospitalized with community-acquired pneumonia, the prevalence of Haemophilus influenzae or Haemophilus haemolyticus was 60.8%, all cases were non-typable H. influenzae (NTHi) or H.
View Article and Find Full Text PDFLancet Child Adolesc Health
March 2024
South African Medical Research Council Vaccines and Infectious Diseases Analytics Research Unit, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa; Wits Infectious Diseases and Oncology Research Institute, University of the Witwatersrand, Faculty of Health Sciences, Johannesburg, South Africa. Electronic address:
Access Microbiol
October 2023
Department of Microbiology and Infectious Diseases, Concord Hospital, NSW Health Pathology, NSW Health, Concord, Australia.
Introduction: In this comparative case study, we discuss clinically relevant discrepancies of antimicrobial susceptibility testing (AST) interpretation for ceftriaxone against a non-typable, beta-lactamase negative, ampicillin-resistant (BLNAR) isolated from a blood culture.
Case Report: A 74-year-old man presented with a 3 day illness characterized by shortness of breath and dry cough, and was noted to be febrile and hypoxic on admission. A blood culture bottle flagged positive with Gram-negative coccobacilli, later identified as with the patient commenced on ceftriaxone.
Acta Microbiol Immunol Hung
December 2023
1Department of Medical Microbiology, Medical Faculty, Medical University of Sofia, 1, G.Sofiiski Boul., 1431-Sofia, Bulgaria.
Haemophilus influenzae is one of the main bacteria responsible for otitis media (OM) among children worldwide. We aimed to estimate the distribution of encapsulated and non-capsulated variants (NTHi), biotypes, antibiotic susceptibility, and molecular epidemiology of H. influenzae isolates recovered from pediatric OM cases in Bulgaria.
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