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Dual-mode colorimetric-fluorescence biosensor for endotoxin detection based on CS@Fe,Cu/CDs-MnO nanomaterials.
Talanta
April 2025
Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, Yunnan, PR China. Electronic address:
Endotoxins are found in the outer membrane of all gram-negative bacteria and are a primary cause of endotoxemia, organ failure, and significant harm to human health. Limulus reagent as traditional detection method has certain limitations for the rapid and accurate detection of endotoxin due to the high costs associated with the horseshoe crab used in the sensing process. Herein, a fluorometric and colorimetric dual-mode biosensor was prepared for the rapid and sensitive detection of endotoxin.
View Article and Find Full Text PDFDual-functional PCN-242 (FeCo) MOF for sensitive bacterial endotoxin detection.
J Mater Chem B
December 2024
Department of Chemistry, National Taiwan Normal University, Taipei 11677, Taiwan.
Endotoxin detection is paramount for monitoring bacterial contamination in food, pharmaceuticals, and clinical diagnostics. The limulus amebocyte lysate (LAL) test, which relies on horseshoe crab blood, has long been the gold standard for endotoxin detection. However, the widespread adoption of this method is constrained by ethical concerns and the high costs associated with harvesting endangered species.
View Article and Find Full Text PDFEndotoxin Detection in Magnetic Resonance Imaging Contrast Agent Using Optimising Chromogenic Limulus Amebocyte Lysate Assay.
Malays J Med Sci
October 2024
Tissue Engineering Group (TEG), National Orthopaedic Centre of Excellence for Research and Learning (NOCERAL), Department of Orthopaedic Surgery, Faculty of Medicine, Universiti Malaya, Kuala Lumpur, Malaysia.
Article Synopsis
- - Endotoxin contamination in MRI contrast agents can lead to immune reactions, raising concerns for patient safety, as there are stricter rules for implants and catheters than for these agents.
- - The study tested a chromogenic LAL assay to measure endotoxin levels in an MRI contrast agent produced in Malaysia, using water for injection to dissolve the powdered agent.
- - Results showed a high coefficient of efficiency (≥ 0.98) and endotoxin levels below the safe limit of 0.5 EU/mL, indicating that the contrast agent is unlikely to cause pyrogenic effects.
Limitations of amebocyte lysate test for endotoxin control in raw materials for liposomal nanoformulations.
Nanomedicine (Lond)
November 2024
Centro de Excelencia en Productos y Procesos (CEPROCOR), Santa María de Punilla, Córdoba, X5164, Argentina.
To evaluate the applicability of amebocyte lysate (LAL) assay for endotoxin determination in lipid compounding liposomal nanoformulations. Spiked cholesterol, hydrogenated soy phosphatidylcholine and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino(polyethylene glycol)-2000] (DSPE-PEG 2000) samples with endotoxins, simulating contaminated samples or in-process contamination were analyzed by chromogenic LAL assay. Recovery of spiked endotoxins was achieved from DSPE-PEG 2000 suspended in water, whereas recovery was not achieved from spiked cholesterol and hydrogenated soy phosphatidylcholine suspended in methanol, and from multilamellar vesicles.
View Article and Find Full Text PDFRecombinant and Synthetic Affibodies Function Comparably for Modulating Protein Release.
Cell Mol Bioeng
August 2024
Department of Bioengineering, University of Oregon, Knight Campus, Eugene, Oregon USA.
Purpose: Affibodies are a class of versatile affinity proteins with a wide variety of therapeutic applications, ranging from contrast agents for imaging to cell-targeting therapeutics. We have identified several affibodies specific to bone morphogenetic protein-2 (BMP-2) with a range of binding affinities and demonstrated the ability to tune release rate of BMP-2 from affibody-conjugated poly(ethylene glycol) maleimide (PEG-mal) hydrogels based on affibody affinity strength. In this work, we compare the purity, structure, and activity of recombinant, bacterially-expressed BMP-2-specific affibodies with affibodies synthesized via solid-phase peptide synthesis.
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