AI Article Synopsis
- The study used whole-cell voltage-clamp techniques on sea urchin oocytes to demonstrate that sperm activation triggers a significant inward current of -521 pA and depolarizes the plasma membrane.
- Simultaneously, the conductance of the membrane increased, indicating a shift in electrical properties due to the sperm's presence.
- Further experiments showed that this inward current is likely calcium dependent, evidenced by the effects of inositol triphosphate and the calcium chelator BAPTA.
Article Abstract
Using the whole-cell voltage-clamp technique to clamp sea urchin oocytes we show that the fertilising spermatozoon triggers an inward current of -521 +/- 56.7 pA (n = 8) at activation. Simultaneously, the plasma membrane depolarises and the conductance increases from 23.4 +/- 1.4 to 40.6 +/- 1.2 nS (n = 8). The I/V curve for the peak activation current is linear and the current reverses between 0 and +20 mV, suggesting a non-specific ion current. Since injection of inositol triphosphate induced an inward current of -1062 +/- 314 pA (n = 4), and the current was inhibited by preloading oocytes with the calcium chelator BAPTA, the non-specific activation current in sea urchin appears to be calcium dependent.
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| http://dx.doi.org/10.1017/s0967199400005037 | DOI Listing |
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