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Serum bta-miRNA-375 as a potential biomarker for the early diagnosis of enzootic bovine leukosis.
PLoS One
May 2024
Faculty of Life and Environmental Sciences, Department of Animal Sciences, Teikyo University of Science, Adachi, Tokyo, Japan.
To identify a biomarker for the early diagnosis of enzootic bovine leukosis (EBL) caused by bovine leukemia virus (BLV), we investigated the expression of a microRNA, bta-miR-375, in cattle serum. Using quantitative reverse-transcriptase PCR analysis, we measured bta-miR-375 levels in 27 samples from cattle with EBL (EBL cattle), 45 samples from animals infected with BLV but showing no clinical signs (NS cattle), and 30 samples from cattle uninfected with BLV (BLV negative cattle). In this study, we also compared the kinetics of bta-miR-375 with those of the conventional biomarkers of proviral load (PVL), lactate dehydrogenase (LDH), and thymidine kinase (TK) from the no-clinical-sign phase until EBL onset in three BLV-infected Japanese black (JB) cattle.
View Article and Find Full Text PDFComparative values of CSF-LDH isoenzymes in neurological disorders.
Indian J Med Sci
January 1999
C.B. Patel Research Centre for Chemistry and Biological Sciences, Bombay.
The present study was carried out to evaluate the usefulness of Cerebrospinal fluid (CSF) Lactate dehydrogenase (LDH) isoenzymes in the diagnosis in tuberculous meningitis (TBM), pyogenic meningitis (PM), viral encephalitis (VE) and hydrocephalus (HC). A characteristic dominance of isoenzymes in cerebrospinal fluid was observed: LDH4 in TBM while LDH3 in PM. However, in VE and HC, LDH2 and LDH1 were dominant respectively.
View Article and Find Full Text PDF[C-reactive protein, lactate and LDH isoenzymes in the cerebrospinal fluid in the diagnosis in childhood meningitis].
An Esp Pediatr
January 1988
Departamento de Pediatría, Hospital General de Galicia, Facultad de Medicina, Universidad de Santiago de Compostela.
C reactive protein lactate and LDH isoenzymes were quantified in CSF of 32 children with meningitis (27 bacterial and 5 viral). Results in cases with bacterial meningitis were as follows. C reactive protein sensitivity was 11.
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