The RNA of satellite tobacco necrosis virus (STNV) is a monocistronic messenger that lacks both a 5' cap and a 3' poly(A) tail. The STNV trailer contains an autonomous translational enhancer domain (TED) that promotes translation in vitro by more than one order of magnitude when combined with the 5'-terminal 173 nt of STNV RNA. We now show that the responsible sequence within the 5' region maps to the first 38 nt of the STNV RNA. Mutational analysis indicated that the primary sequence of the STNV 5' 38 nt and TED is important for translation stimulation in vitro, but did not reveal a role for the complementarity between the two. Translation of chimeric STNV-cat RNAs in tobacco protoplasts showed that TED promotes translation in vivo of RNAs lacking a cap and/or a poly(A) tail. Similar to in vitro, TED-dependent translation in tobacco was stimulated further by the STNV 5' 38 nt.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1046/j.1365-313x.1998.00104.x | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Characterisation of a Betasatellite Associated With Tomato Yellow Leaf Curl Guangdong Virus and Discovery of an Unusual Modulation of Virus Infection Associated With C4 Protein.
Mol Plant Pathol
January 2025
Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, China.
Tomato yellow leaf curl Guangdong virus (TYLCGdV), a monopartite begomovirus first identified in 2004, remains poorly characterised. In this study, we demonstrate that TYLCGdV associates with a betasatellite, TYLCGdB, and the βC1 protein encoded by TYLCGdB is essential for symptom development. We also explore the role of TYLCGdV C4 protein by generating a C4-deficient infectious clone (TYLCGdV), revealing a dynamic role for TYLCGdV C4.
View Article and Find Full Text PDFAccurately Segmenting/Mapping Tobacco Seedlings Using UAV RGB Images Collected from Different Geomorphic Zones and Different Semantic Segmentation Models.
Plants (Basel)
November 2024
School of Geography & Environmental Sciences, Guizhou Normal University, Guiyang 500025, China.
The tobacco seedling stage is a crucial period for tobacco cultivation. Accurately extracting tobacco seedlings from satellite images can effectively assist farmers in replanting, precise fertilization, and subsequent yield estimation. However, in complex Karst mountainous areas, it is extremely challenging to accurately segment tobacco plants due to a variety of factors, such as the topography, the planting environment, and difficulties in obtaining high-resolution image data.
View Article and Find Full Text PDFThe complete genome assembly of Nicotiana benthamiana reveals the genetic and epigenetic landscape of centromeres.
Nat Plants
December 2024
Shandong Key Laboratory of Precision Molecular Crop Design and Breeding, Peking University Institute of Advanced Agricultural Sciences, Shandong Laboratory of Advanced Agricultural Sciences in Weifang, Weifang, China.
Article Synopsis
- Nicotiana benthamiana is a key model organism in plant biology, and researchers have generated a complete genome assembly for the plant, which is 2.85 Gb in size.
- The study reveals that while Ty3/Gypsy retrotransposons are common in centromeres of the Solanaceae family, N. benthamiana surprisingly has many centromeres rich in satellite DNA, which are essential for their function.
- The findings suggest that new centromeres in N. benthamiana may form through processes involving neocentromere formation, satellite expansion, and the integration of mitochondrial DNA.
Coordinate-based simulation of pair distance distribution functions for small and large molecular assemblies: implementation and applications.
J Appl Crystallogr
October 2024
Chemical Sciences and Engineering Division Argonne National Laboratory Lemont IllinoisUSA.
X-ray scattering has become a major tool in the structural characterization of nanoscale materials. Thanks to the widely available experimental and computational atomic models, coordinate-based X-ray scattering simulation has played a crucial role in data interpretation in the past two decades. However, simulation of real-space pair distance distribution functions (PDDFs) from small- and wide-angle X-ray scattering, SAXS/WAXS, has been relatively less exploited.
View Article and Find Full Text PDFArticle Synopsis
- * Recent efforts to stop smoking haven't been put into action yet, and it’s important to see what could happen if smoking rates stay the same or improve.
- * Researchers used models to predict health outcomes by 2050 based on different scenarios of smoking rates, showing that cutting smoking could greatly improve health and life expectancy.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!