AI Article Synopsis
- The study explored how lymphocytes from diabetic mice interact with the pancreatic and gastric tissues of nondiabetic mice using in vivo microscopy.
- The transferred diabetic lymphocytes showed significantly higher rolling and adhesion rates in the islets of nondiabetic mice that were pre-treated with a low dose of streptozotocin, compared to non-treated mice or those receiving lymphocytes from nondiabetic donors.
- The findings suggest that diabetic donor lymphocytes preferentially target islet endothelium, but additional factors are required for effective adhesion to the islets in nondiabetic recipients.
Article Abstract
The interaction between intravenously transferred lymphocytes derived from spleens of multiple low-dose streptozotocin-diabetic mice with islet, exocrine pancreatic, and gastric mucosal endothelium of nondiabetic recipient mice was investigated by in vivo microscopy. Donor lymphocytes were stained with acridine red in vitro. The adoptive transfer of these cells from diabetic donor animals resulted in significantly increased lymphocyte rolling (4.46 +/- 1.32%, P < 0.05) and adhesion (3.86 +/- 1.04%, P < 0.05) in islets of nondiabetic recipients that had been pretreated with a single subdiabetogenic dose of streptozotocin. No increased endothelial interaction was noted in nonpretreated recipients or in experiments with nondiabetic donors. Rolling (1.19 +/- 0.61 to 2.71 +/- 0.62%) and adhesion (0.61 +/- 0.33 to 2.80 +/- 0.97%) of donor lymphocytes were low in exocrine pancreatic and gastric mucosal control tissue. It is concluded that, in this animal model, lymphocytes from diabetic donors interact preferentially with recipient islet endothelium. However, additional stimulation of recipient islet endothelium by exogenous factors is necessary to enable transferred cells to adhere to pancreatic islets.
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| http://dx.doi.org/10.1152/ajpendo.1998.274.5.E928 | DOI Listing |
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