A fingerprinting technique similar to repetitive extragenic palindromic PCR was developed to identify strains of Lactococcus lactis. The method distinguishes closely related strains and discriminates among some with identical ldh sequences. The fingerprinting primer LL-Rep1 complements a moderately repeated sequence found in low G + C Gram-positive bacteria and may therefore prove useful for discriminating among strains of other low G + C Gram-positive species.
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http://dx.doi.org/10.1111/j.1574-6968.1998.tb12986.x | DOI Listing |
Food Addit Contam Part A Chem Anal Control Expo Risk Assess
January 2025
UMR SayFood 0782, Université Paris-Saclay, INRAE, Palaiseau, AgroParisTech, France.
Assessing the contamination of paper and board (P&B) food packaging materials poses significant challenges due to the sensitivity limits of analytical methods and the low precision of sampling processes. This study aims to enhance the understanding of P&B food packaging contamination by investigating the distribution of contaminants at different scales using a combination of chromatographic and spectroscopic techniques. A total of 36 substances were targeted, including phthalates, photoinitiators, and bisphenol A.
View Article and Find Full Text PDFAnal Methods
January 2025
Pharmacognosy Division, CSIR-National Botanical Research Institute, Lucknow, UP, 226001, India.
This work deals with the development of a greener RP-HPLC method and chemical pattern recognition for the identification of L. collected from different natural sources and samples traded as '' in Indian herbal drug markets. The simultaneous quantification of α- and β-asarone was performed using 0.
View Article and Find Full Text PDFAnal Methods
January 2025
Shaanxi Province Key Laboratory of New Drugs and Chinese Medicine Foundation Research, Pharmacy College, Shaanxi University of Chinese Medicine, No. 1, Shiji Avenue, Xi Xian New District, Xi'an City, 712046 Xianyang, Shaanxi Province, China.
Aim: this study aimed to examine the effect of different storage times (0, 7, 24, 57, and 119 days) on the volatile components of Shenling Baizhu powder across different preparation processes (Pharmacopoeia, ultra-micro pulverization-pulverization, and microparticle design methods). The findings offer insights to guide quality control measures for Shenling Baizhu powder.
Methods: gas chromatography-ion mobility spectrometry (GC-IMS) was employed to ascertain the volatile components in Shenling Baizhu powder at various storage times across different preparation processes.
Biosensors (Basel)
January 2025
College of Optical and Electronic Technology, China Jiliang University, Hangzhou 310018, China.
Nucleic acid aptamers are single-stranded oligonucleotides that are selected through exponential enrichment (SELEX) technology from synthetic DNA/RNA libraries. These aptamers can specifically recognize and bind to target molecules, serving as specific recognition elements. Surface-enhanced Raman scattering (SERS) spectroscopy is an ultra-sensitive, non-destructive analytical technique that can rapidly acquire the "fingerprint information" of the measured molecules.
View Article and Find Full Text PDFJ Chromatogr Sci
January 2025
Chemical Technology Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur 176061, HP, India.
Adulteration and illegal trade of Saussurea lappa in the name of Inula racemosa is a major issue. Therefore, accurate and easy methods are required to control malpractice and define authenticity. The current study is focused on authenticating and defining quality control methods for I.
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!