The dependence of urokinase (uPA) secretion on basic fibroblast growth factor (bFGF) and platelet-deprived growth factor (PDGF BB) was investigated by using of cultured rat aortic smooth muscle cells (SMC). Growth factors stimulated secretion of urokinase with two-phase kinetics within 1-60 minutes. Namely, "apparent" concentration of uPA in the conditioned media rised to 12.5 nM within the first 1-2 min and 5.8 nM after 30 min of cell stimulation by growth factors, and decreased to basal level at 5 min after stimulation of the cells. The character of uPA-secretion kinetics was similar in response to both growth factors, but the level of secreted uPA was higher in case of PDGF BB. We have shown that this decrease of uPA content in conditioned media is not related to the binding of uPA to the cell surface receptors or extracellular matrix proteins. One can suppose that urokinase secreted within 5 minutes could bind to secretory protein which nature has to be identified. But it was established that this secretory protein, complexing urokinase in cultured media, is not identical to the plasminogen activator inhibitor type 1 (PAI-1).
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
TOM40 as a prognostic oncogene for oral squamous cell carcinoma prognosis.
BMC Cancer
January 2025
Department of Otorhinolaryngology, Shenzhen Key Laboratory of Otorhinolaryngology, Longgang Otorhinolaryngology Hospital, Shenzhen Institute of Otorhinolaryngology, No. 3004 Longgang Avenue, Shenzhen, Guangdong, China.
Background: To investigate the role of the translocase of the outer mitochondrial membrane 40 (TOM40) in oral squamous cell carcinoma (OSCC) with the aim of identifying new biomarkers or potential therapeutic targets.
Methods: TOM40 expression level in OSCC was evaluated using datasets downloaded from The Cancer Genome Atlas (TCGA), as well as clinical data. The correlation between TOM40 expression level and the clinicopathological parameters and survival were analyzed in TCGA.
Preterm preeclampsia screening and prevention: a comprehensive approach to implementation in a real-world setting.
BMC Pregnancy Childbirth
January 2025
Genetic Program, North York General Hospital, Toronto, ON, Canada.
Background: Preeclampsia significantly impacts maternal and perinatal health. Early screening using advanced models and primary prevention with low-dose acetylsalicylic acid for high-risk populations is crucial to reduce the disease's incidence. This study assesses the feasibility of implementing preterm preeclampsia screening and prevention by leveraging information from our current aneuploidy screening program in a real-world setting with geographic separation clinical site and laboratory analysis site.
View Article and Find Full Text PDFAge-period-cohort analysis of self-harm incidence rates by gender in Iran among individuals aged 10-39 years, 1990-2019.
Sci Rep
January 2025
Family Health and Population Growth Research Center, Health Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran.
This study investigates trends and patterns in self-harm incidence rates among individuals aged 10-39 years in Iran from 1990 to 2019. Using Age-Period-Cohort (APC) analysis, it aims to identify significant age, period, and cohort effects, and to highlight gender-specific trends for effective prevention strategies. Utilizing data from the Global Burden of Disease (GBD) study, we performed an APC analysis to assess trends in self-harm incidence rates by gender.
View Article and Find Full Text PDFAnalysis of changes in the chemokine CXC ligand 13 in serum and cerebrospinal fluid of patients with neuromyelitis optica.
Sci Rep
January 2025
Department of Neurology, Chenzhou First People's Hospital, Chenzhou City, 423000, Hunan Province, China.
To determine correlation between the Extended Disability Status Scale(EDSS) grade and the progression of neuromyelitis optica(NMO) patients' levels of the chemokine CXC ligand 13 (CXCL13) in their serum and cerebrospinal fluid. This research included forty-one patients diagnosed with neuromyelitis optica(NMO) and forty-three patients diagnosed with multiple sclerosis(MS). The control group consisted of forty-three non-inflammatory neurological disease(NND) patients.
View Article and Find Full Text PDFSpaciotemporal distribution characteristics of glacial lakes and the factors influencing the Southeast Tibetan Plateau from 1993 to 2023.
Sci Rep
January 2025
College of Water Conservancy and Civil Engineering, Tibet Agriculture and Animal Husbandry University, Nyingchi, 860000, China.
The southeastern region of Tibet, which serves as the primary concentration area for marine-type glaciers, has fostered a multitude of glacial lakes that are highly sensitive to global climate change. Glacial lakes play a crucial role in regulating the freshwater ecosystems of the region, but they also pose a significant threat to local infrastructure and populations due to flooding caused by glacial lake outbursts. Currently, a limited amount of research has focused on the monitoring and analysis of glacial lakes in southeastern Tibet.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!