Differentiation of Cryptosporidium parvum isolates by a simplified randomly amplified polymorphic DNA technique.

Appl Environ Microbiol

Department of Population Health and Reproduction, School of Veterinary Medicine, University of California-Davis 95616, USA.

Published: May 1998

Genomic DNA was isolated from Cryptosporidium parvum oocysts by a specific immunomagnetic separation-in vitro excystation procedure and subjected to randomly amplified polymorphic DNA analysis using sequence-independent primers. An estuary C. parvum isolate was easily differentiated from several bovine isolates, while five bovine isolates of the same origin were indistinguishable from each other.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC106259PMC
http://dx.doi.org/10.1128/AEM.64.5.1954-1957.1998DOI Listing

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