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Assembly and Quantification of Co-Cultures Combining Heterotrophic Yeast with Phototrophic Sugar-Secreting Cyanobacteria.
J Vis Exp
December 2024
Institute for Synthetic Microbiology, Heinrich Heine University Düsseldorf; Department of Biochemistry and Molecular Biology, Michigan State University.
With the increasing demand for sustainable biotechnologies, mixed consortia containing a phototrophic microbe and heterotrophic partner species are being explored as a method for solar-driven bioproduction. One approach involves the use of CO2-fixing cyanobacteria that secrete organic carbon to support the metabolism of a co-cultivated heterotroph, which in turn transforms the carbon into higher-value goods or services. In this protocol, a technical description to assist the experimentalist in the establishment of a co-culture combining a sucrose-secreting cyanobacterial strain with a fungal partner(s), as represented by model yeast species, is provided.
View Article and Find Full Text PDFNavigating Artificial Intelligence in Scientific Manuscript Writing: Tips and Traps.
Indian J Radiol Imaging
January 2025
Department of Radiodiagnosis and Imaging, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India.
It is being increasingly recognized that the strategic use of artificial intelligence (AI) can catalyze the process of manuscript writing. However, it is imperative that we recognize the hidden biases, pitfalls, and disadvantages of relying solely on AI, such as accuracy concerns and the potential erosion of nuanced human insight. With an emphasis on crafting effective prompts and inputs, this article reveals how to navigate the labyrinth of AI capabilities to create a good-quality manuscript.
View Article and Find Full Text PDFCerebrovascular thrombosis is among the most critical medical conditions, making early diagnosis and management crucial. Although some symptoms of cerebrovascular thrombosis are typical and lead to early diagnosis, they can sometimes present with rare and unusual symptoms, complicating the diagnostic process. Given the morbidity and mortality associated with these events, it is important to be aware of unexpected symptoms to diagnose and manage these patients more accurately and rapidly.
View Article and Find Full Text PDF'Choosing Tuesday': establishing and sustaining regular clinical supervision in advanced nursing practice.
Br J Nurs
January 2025
Registered Advanced Nurse Practitioner, Emergency Department, St James's Hospital, Dublin.
Clinical supervision is a valued learning tool for student nurses; however, there is a paucity of description around real-time experience of clinical supervision among qualified advanced nurse practitioners. Many qualified nurses claim delays in engaging with clinical supervision may be caused by staff shortages, time constraints, workload in busy clinical environments, or a reticence to engage in discussions that might reveal shortcomings in knowledge or practical skills. This article reviews a process of monthly clinical supervision that has been conducted among a group of qualified emergency department advanced nurse practitioners for 25 years.
View Article and Find Full Text PDFSeasonal Distribution and Diversity of Non-Insect Arthropods in Arid Ecosystems: A Case Study from the King Abdulaziz Royal Reserve, Kingdom Saudi Arabia.
Biology (Basel)
December 2024
Department of Zoology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.
The biodiversity of invertebrate animals is largely affected by climatic changes. This study evaluates the seasonal abundance and diversity of non-insect arthropods in the King Abdulaziz Royal Reserve (KARR), Saudi Arabia, over four collection periods (summer, autumn, winter, and spring) during 2023. Sampling was conducted across multiple sites in the reserve using both active (manual collection and active surveying for the diurnal species) and passive (pitfall traps and malaise traps for the nocturnal species) methods.
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