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Background: Laparoscopic surgery training is a demanding process requiring technical and nontechnical skills. Surgical training has evolved from traditional approaches to the use of immersive digital technologies such as virtual, augmented, and mixed reality. These technologies are now integral to laparoscopic surgery training.

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Crystal structure of submicron-sized sulfur particles using 3D ED obtained in atmospheric conditions.

Acta Crystallogr C Struct Chem

February 2025

Electron Microscopy for Materials Science (EMAT), University of Antwerp, Groenenborgerlaan 171, Antwerp, 2020, Belgium.

Lithium-sulfur batteries are a promising candidate for the next generation of rechargeable batteries. Despite extensive research on this system over the last decade, a complete understanding of the phase transformations has remained elusive. Conventional in-situ powder X-ray diffraction has struggled to determine the unit cell and space group of the polysulfides formed during charge and discharge cycles due to the high solubility of these solid products in the liquid electrolyte.

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Targeted barcoding of variable antibody domains and individual transcriptomes of the human B-cell repertoire using Link-Seq.

PNAS Nexus

January 2025

Institute of Bioengineering, School of Engineering, École Polytechnique Fédérale de Lausanne (EPFL), 1015 Lausanne, Switzerland.

Here, we present Link-Seq, a highly efficient droplet microfluidic method for combined sequencing of antibody-encoding genes and the transcriptome of individual B cells at large scale. The method is based on 3' barcoding of the transcriptome and subsequent single-molecule PCR in droplets, which freely shift the barcode along specific gene regions, such as the antibody heavy- and light-chain genes. Using the immune repertoire of COVID-19 patients and healthy donors as a model system, we obtain up to 91.

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Selective activity of Tabebuia avellanedae against Giardia duodenalis infecting organoid-derived human gastrointestinal epithelia.

Int J Parasitol Drugs Drug Resist

January 2025

Department of Infectious Diseases, Unit of Foodborne and Neglected Parasitic Diseases, Istituto Superiore di Sanità, viale Regina Elena 299, Rome, Italy. Electronic address:

Giardia duodenalis is a widespread intestinal protozoan that affects mammals, including humans. Symptoms can range from being subclinical to causing severe abdominal pain and diarrhoea. Giardiasis often requires repeated treatment with synthetic drugs like metronidazole.

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Eicosapentaenoic Acid and Docosahexaenoic Acid as an Antimicrobial Agent in Orthopedics-An In Vitro Study About the Race for Surface.

Pathogens

January 2025

Research Laboratory for Biofilms and Implant Associated Infections (BIOFILM LAB), University Hospital for Orthopaedics and Traumatology, Medical University of Innsbruck, Müllerstraße 44, 6020 Innsbruck, Austria.

Background: The burden of prosthetic joint infection in combination with antibiotic-resistant bacterial strains is a rising dilemma for patients experiencing total joint replacements. Around 0.8-2% of patients experience prosthetic joint infections, while up to 21% of patients are considered fatal cases after 5 years.

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