A comparison of the high resolution mass spectra of synthetic and natural preparations of malformin provided new evidence for the revised structure: (see article): The interpretation of the mass spectra was aided by the examination of the spectra of the synthetic peptides, desthiomalformin and enantio-5-valine malformin.
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http://dx.doi.org/10.7164/antibiotics.29.549 | DOI Listing |
J Clin Microbiol
January 2025
Department of Mycobacteriology, Leprosy Research Center, National Institute of Infectious Diseases, Higashimurayama, Tokyo, Japan.
, a slow-growing nontuberculous mycobacterium, causes Buruli ulcer, a neglected tropical disease. Distinguishing from related species, including , poses challenges with respect to making accurate identifications. In this study, we developed a rapid and simple identification method based on mycobacterial lipid profiles and used matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) to analyze the lipid profiles of ( = 35) and ( = 19) isolates.
View Article and Find Full Text PDFFront Microbiol
January 2025
Research Institute of International Agriculture, Technology and Information, Hankyong National University, Anseong-si, Republic of Korea.
Volatile organic compounds (VOCs) produced by potential plant growth-promoting rhizobacteria (PGPR) play an important role in plant interactions. However, the mechanisms underlying this phenomenon are not well understood. Our findings show that the influence of VOCs from the PGPR strain (EXTN-1) on tobacco plant growth is dependent on the culture media used.
View Article and Find Full Text PDFJ Chromatogr A
January 2025
Centro de Metabolómica y Bioanálisis (CEMBIO), Facultad de Farmacia, Universidad San Pablo-CEU, CEU Universities, Urbanización Montepríncipe, Boadilla del Monte 28660, España. Electronic address:
Mammalian hibernation offers a unique model for exploring neuroprotective mechanisms relevant to neurodegenerative diseases. In this study, we employed untargeted lipidomics with iterative tandem mass spectrometry (MS/MS) to profile the brain lipidome of Syrian hamsters across different hibernation stages: late torpor, arousal, and euthermia (control). Previously, a lipid species identified as methyl-PA(16:0/0:0) showed a significant increase during torpor, but its precise structure was unresolved due to technological constraints.
View Article and Find Full Text PDFInt J Biol Macromol
January 2025
Core Facility Center "Arktika", Northern (Arctic) Federal University named after M.V. Lomonosov, Northern Dvina Emb., 17, Arkhangelsk 163002, Russian Federation. Electronic address:
Dioxane lignin (DL) is isolated from plant material under mild acidolysis conditions and is widely used in many studies as a representative sample of protolignin, an alternative to milled wood lignin (MWL). However, the structural changes caused by hydrolytic degradation reactions during DL extraction are still poorly understood. In this work, an integrated approach based on 2D NMR and high-resolution mass spectrometry was used to establish the features of the lignin structure on the example of pine lignin isolated using dioxane under various conditions: MWL, DL and "formaldehyde stabilized" lignin (LSF).
View Article and Find Full Text PDFPlants (Basel)
January 2025
School of Agriculture, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.
Plant factories with artificial lighting (PFALs) are a notable choice for urban agriculture due to the system's benefits, where light can be manipulated to enhance the product's yield and quality. Our objective was to test the effect of light spectra with different red-blue combinations and white light on the growth, physiology, and overall quality of three baby-leaf vegetables (green lettuce, kale, and pak choi) grown in a restaurant's PFAL. Leaf mass per area was lower under the most blue-containing treatments in all species.
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