Background/aims: Reactive oxygen species play an essential role in necro-inflammatory processes. Therefore, the aim of the present studies was to investigate the effect of exogenous and endogenously produced H2O2 on the phagocytic capacity and glucose release of perfused cirrhotic rat livers in comparison with that on the controls.
Methods: Complete septal cirrhosis was achieved by oral treatment of rats with thioacetamide for 6 months. The phagocytic capacity of the perfused livers was measured by the uptake of colloidal carbon. During the continuous perfusion with colloidal carbon, either H2O2 or benzylamine was added to the perfusion medium for a limited time period. The latter functioned as an endogenous H2O2 donor.
Results: In control rats exogenous and endogenously produced H2O2 caused a transient stimulation of the hepatic colloidal carbon uptake as well as of the glucose release. Inhibition of the catalase by aminotriazol doubled the changes evoked by H2O2, whereas blockade of the Kupffer cells by GdCl3 drastically reduced its stimulatory effect. Cirrhotic livers took up less colloidal carbon and released lower amounts of glucose than the controls when stimulated by exogenous H2O2. The inhibition of the nitric oxide synthetase augmented the H2O2-induced effect in controls as well as in the cirrhotic livers by 250% and 620% (colloidal carbon uptake) and 340% and 760% (glucose release), respectively. The blockade of the eicosanoid production by indomethacin and caffeic acid drastically increased the glucose release and the colloidal carbon uptake in controls and, in absolute terms, to a lesser extent in cirrhotic livers. Endogenous H2O2 produced by the addition of benzylamine stimulated the colloidal carbon uptake and glucose release in livers from both groups. The inhibition of the lipoxygenase increased both parameters, whereas different effects were elicited by the addition of superoxide dismutase in controls and cirrhotic livers.
Conclusion: The maximum uptake of colloidal carbon and glucose release, measured after stimulation by H2O2, was lower in cirrhotic livers than in controls, thus indicating a lowered phagocytic capacity of Kupffer cells and altered glycogenolytic response of the hepatocytes in cirrhotic livers. The use of various effectors provided evidence that superoxide anions, nitric oxide and, possibly, arachidonic acid are involved in the signal transduction between Kupffer cells and hepatocytes when stimulated by exogenous or endogenously produced H2O2. This signalling mechanism seems to be impaired in cirrhotic livers.
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http://dx.doi.org/10.1016/s0168-8278(98)80321-8 | DOI Listing |
J Colloid Interface Sci
January 2025
Department of Chemistry, Southern University of Science and Technology, Shenzhen 518055 China. Electronic address:
In the typical ionothermal synthesis of crystalline carbon nitride (CCN), alkali metal halides are usually used in large amounts. Here, we report a new method for synthesizing poly (heptazine imide) (PHI) using only a trace amount of NaF, which is 20 times less than the amount of NaCl typically required to achieve the PHI structure. Different from the prevailing view that salts function primarily as templates and chelating agents during polymerization, our research revealed the unique role that NaF plays in the polymerization of PHI.
View Article and Find Full Text PDFJ Colloid Interface Sci
January 2025
Yanshan Earth Critical Zone and Surface Fluxes Research Station, College of Resources and Environment, University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address:
Periodate (PI) activation via three-dimensional electrochemical (E) is a promising approach for degrading sulfisoxazole (SIZ), while the scarcity of active sites significantly limits the efficient electron-transfer rate. Herein, we synthesized multiple strongly active zero-valent cobalt (Co) nanoparticles encapsulated in nitrogen-doped carbon (NC) shells through Co-potassium chloride (KCl) doping pyrolysis of Zeolitic Imidazolate Framework-8 (ZIF-8) to induce the rapid electron transfer pathways (ETP). Specifically, molten KCl doping provides confined structures for Co with a diameter of 12.
View Article and Find Full Text PDFAdv Mater
January 2025
Institute of Functional Nano & Soft Materials (FUNSOM), Jiangsu Key Laboratory of Advanced Negative Carbon Technologies, Soochow University, Suzhou, 215123, China.
Formamidinium lead iodide (FAPbI) perovskite films, ensuring optically active phase purity with uniform crystal orientation, are ideal for photovoltaic applications. However, the optically active α-FAPbI phase is easy to degrade into δ-phase due to numerous defects within randomly oriented films. Here, a "quasi-2D" perovskite template is pre-deposited on the film surface within the crystallization process based on the two-step preparation technology, which directly induced pure and highly orientated crystallization of α-FAPbI across the downward growth process.
View Article and Find Full Text PDFJ Colloid Interface Sci
January 2025
Henan Key Laboratory of Polyoxometalate Chemistry, School of Energy Science and Technology, Henan University, Zhengzhou 450046, PR China. Electronic address:
Due to the limited active sites and poor conductivity, the application of tungsten disulfide (WS) in alkaline water electrolysis remains a challenge. Herein, Ni-WS nanosheet arrays were in situ grown on the carbon fiber paper (Ni-WS/CFP) as an electrocatalyst for hydrogen evolution reaction (HER) and oxygen evolution reaction (OER) in alkaline media, and the introduction degree of Ni can be regulated by adjusting the electrodeposition time. When the electrodeposition time is 3 min, Ni ions are doped into the lattice of WS, and by prolonging the electrodeposition time to 10 min, the nickel disulfide (NiS) crystal phase is generated to form NiS@WS heterojunction.
View Article and Find Full Text PDFJ Colloid Interface Sci
January 2025
CAS Key Laboratory of Carbon Materials, Institute of Coal Chemistry, Chinese Academy of Sciences, Taiyuan 030001, China. Electronic address:
The development of advanced bifunctional oxygen electrocatalysts for the oxygen reduction reactions (ORR) and oxygen evolution reactions (OER) is crucial for the practical application of zinc-air batteries (ZABs). Herein, porous carbon nanosheets integrated with abundant graphene-wrapped CoO and CoNx (CoO/CoNx-C) were successfully fabricated through a simple one-step pyrolysis. With convenient porous channel and large accessible surface, abundant CoO/CoNx species and graphene wrapping structure, CoO/CoNx-C exhibited a half-wave potential of 0.
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