Purpose: To investigate donor cell survival following corneal limbal stem cell grafting, which is based on the corneal stem cell model.
Methods: We describe the use of the amelogenin gene probe with the polymerase chain reaction (PCR) to detect surviving donor cells and report preliminary studies using Y-specific DNA probes.
Results And Conclusions: DNA polymorphisms have a detection limit of 10%. The SRY 'Y-specific' probe has a theoretical detection limit of 1 cell in 10,000. The techniques were applied to investigate survival of male donor cells in an aniridic female patient 2 1/2 years following limbal stem cell grafts. We speculate that low levels of donor-derived cells may still be present. We discuss the advantages and disadvantages of the two approaches, which may have future clinical and experimental application.
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| http://dx.doi.org/10.1038/eye.1997.204 | DOI Listing |
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