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Modular cloning of multigene vectors for the baculovirus system and yeast.
J Mol Biol
January 2025
Department of Biochemistry and Molecular Biology, Biomedicine Discovery Institute, Faculty of Medicine, Nursing and Health Sciences, Monash University Clayton Victoria Australia; EMBL-Australia Clayton Victoria Australia. Electronic address:
Recombinant macromolecular complexes are often produced by the baculovirus system, using multigene expression vectors. Yet, the construction of baculovirus-compatible multigene expression vectors is complicated and time-consuming. Furthermore, while the baculovirus and yeast are popular protein expression systems, no single method for multigene vector construction is compatible with both.
View Article and Find Full Text PDFApplying phylogenetic methods for species delimitation to distinguish B-cell clonal families.
Front Immunol
December 2024
Department of Quantitative and Computational Biology, University of Southern California, Los Angeles, CA, United States.
The adaptive immune system generates a diverse array of B-cell receptors through the processes of V(D)J recombination and somatic hypermutation. B-cell receptors that bind to an antigen will undergo clonal expansion, creating a Darwinian evolutionary dynamic within individuals. A key step in studying these dynamics is to identify sequences derived from the same ancestral V(D)J recombination event (i.
View Article and Find Full Text PDFFunctional Analysis of and Related to Floral Terpenoids Biosynthesis in Tree Peony.
Int J Mol Sci
November 2024
College of Landscape Architecture, Beijing University of Agriculture, Beijing 102206, China.
Tree peony (), as a popular ornamental plant worldwide, has a unique floral fragrance, and it is important in the pollination, ornamental, food, and fragrance product industries. However, the underlying molecular mechanisms for the synthesis of floral fragrance terpenoids in tree peony are not well understood, constraining their exploitation. 'Oukan' produces strong floral fragrance terpenoids with high ornamental value and excellent stress resistance and is considered a valuable model for studying tree peony floral fragrance formation.
View Article and Find Full Text PDFGrowth of Renal Cancer Cell Lines Is Strongly Inhibited by Synergistic Activity of Low-Dosed Amygdalin and Sulforaphane.
Nutrients
October 2024
Department of Urology and Pediatric Urology, University Medical Center Mainz, 55131 Mainz, Germany.
: Plant derived isolated compounds or extracts enjoy great popularity among cancer patients, although knowledge about their mode of action is unclear. The present study investigated whether the combination of two herbal drugs, the cyanogenic diglucoside amygdalin and the isothiocyanate sulforaphane (SFN), influences growth and proliferation of renal cell carcinoma (RCC) cell lines. : A498, Caki-1, and KTCTL-26 cells were exposed to low-dosed amygdalin (1 or 5 mg/mL), or SFN (5 µM) or to combined SFN-amygdalin.
View Article and Find Full Text PDFSingle-Cell Transcriptomic Analysis of Normal and Malignant B Cells.
Methods Mol Biol
October 2024
Institute for Cancer Genetics and Department of Pathology & Cell Biology, Columbia University, New York, NY, USA.
In the past decade, single-cell (sc) transcriptomics has overcome the limitations of bulk analysis by measuring gene expression in individual cells, not just a population average. This can identify diverse cell types and states within a sample with high resolution, even without prior purification. Various technologies exist, each with its own capture, barcoding, and library preparation methods.
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