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Unlocking regeneration potential: harnessing morphogenic regulators and small peptides for enhanced plant engineering.
Plant J
January 2025
Department of Agronomy, Iowa State University, Ames, Iowa, 50011, USA.
Plant genetic transformation is essential for understanding gene functions and developing improved crop varieties. Traditional methods, often genotype-dependent, are limited by plants' recalcitrance to gene delivery and low regeneration capacity. To overcome these limitations, new approaches have emerged that greatly improve efficiency and genotype flexibility.
View Article and Find Full Text PDFPhotothermal Nanomaterial-Mediated Photoporation.
Acc Chem Res
March 2023
Joint Laboratory of Advanced Biomedical Materials (NFU-UGent), Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, Nanjing Forestry University (NFU), No. 159 Longpan Road, Nanjing 210037, China.
Delivering biological effector molecules in cultured cells is of fundamental importance to any study or application in which the modulation of gene expression is required. Examples range from generating engineered cell lines for studying gene function to the engineering of cells for cell-based therapies such as CAR-T cells and gene-corrected stem cells for regenerative medicine. It remains a great challenge, however, to deliver biological effector molecules across the cell membrane with minimal adverse effects on cell viability and functionality.
View Article and Find Full Text PDFBiolistic delivery of liposomes protected in metal-organic frameworks.
Proc Natl Acad Sci U S A
March 2023
Department of Chemistry and Biochemistry, University of Texas at Dallas, Richardson, TX 75080-3021.
Needle-and-syringe-based delivery has been the commercial standard for vaccine administration to date. With worsening medical personnel availability, increasing biohazard waste production, and the possibility of cross-contamination, we explore the possibility of biolistic delivery as an alternate skin-based delivery route. Delicate formulations like liposomes are inherently unsuitable for this delivery model as they are fragile biomaterials incapable of withstanding shear stress and are exceedingly difficult to formulate as a lyophilized powder for room temperature storage.
View Article and Find Full Text PDFCarrier gas triggered controlled biolistic delivery of DNA and protein therapeutics from metal-organic frameworks.
Chem Sci
November 2022
Department of Chemistry and Biochemistry, The University of Texas at Dallas 800 West Campbel Rd Richardson 75080 TX USA
The efficacy and specificity of protein, DNA, and RNA-based drugs make them popular in the clinic; however, these drugs are often delivered injection, requiring skilled medical personnel, and producing biohazardous waste. Here, we report an approach that allows for their controlled delivery, affording either a burst or slow release without altering the formulation. We show that when encapsulated within zeolitic-imidazolate framework eight (ZIF-8), the biomolecules are stable in powder formulations and can be inoculated with a low-cost, gas-powered "MOF-Jet" into living animal and plant tissues.
View Article and Find Full Text PDFIdentifying Factors that Determine Effectiveness of Delivery Agents in Biolistic Delivery Using a Library of Amine-Containing Molecules.
ACS Appl Bio Mater
October 2022
Department of Materials Science and Engineering, Iowa State University, Ames, Iowa 50011, United States.
Biolistic transfection is a popular and versatile tool for plant transformation. A key step in the biolistic process is the binding of DNA to the heavy microprojectile using a delivery agent, usually a positively charged molecule containing amine groups. Currently, the choice of the commercial delivery agent is mostly limited to spermidine.
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