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A Highly Efficient System for Separating Glandular and Non-glandular Trichome of Cucumber Fruit for Transcriptomic and Metabolomic Analysis.
Bio Protoc
January 2025
Department of Vegetable Science, College of Horticulture, China Agricultural University, Beijing, China.
Cucumber () trichomes play a critical role in resisting external biological and abiotic stresses. Glandular trichomes are particularly significant as they serve as sites for the synthesis and secretion of secondary metabolites, while non-glandular trichomes are pivotal for determining the appearance quality of cucumbers. However, current methods for separating trichomes encounter challenges such as low efficiency and insufficient accuracy, limiting their applicability in multi-omics sequencing studies.
View Article and Find Full Text PDFInfluence of Parameters Used to Prepare Sterile Solutions of Poloxamer 188 on Their Physicochemical Properties.
Polymers (Basel)
December 2024
Institute of Theoretical and Experimental Biophysics RAS, Pushchino 142290, Russia.
The physicochemical properties of emulsions based on poloxamers (triblock copolymers of a hydrophobic polyoxypropylene chain and two hydrophilic polyoxyethylene chains) depend on the composition and preparation method. This study examined the impact of poloxamer P188 concentration, autoclaving mode, heating, and salt presence on the viscosity, particle size distribution, and morphology of particles using viscometric analysis, dynamic light scattering (DLS), and atomic force microscopy (AFM). It was shown that sample preparation affects the particle size and morphology but not the chemical composition of P188.
View Article and Find Full Text PDFDevelopment of a 50% (w/v) aluminium chloride hexahydrate solution as haemostatic agent.
Eur J Hosp Pharm
January 2025
Pharmacy Department, Hospices Civils de Lyon, Groupement Hospitalier Nord, Lyon, France
Objectives: Acceleration of the haemostasis process after dermatological surgery predominantly relies on mechanical methods, such as the use of sutures or staples. To our knowledge, there is currently no commercialised haemostatic agent for this specific application. Due to the protein precipitation properties of the 50% (w/v) aluminium chloride hexahydrate solution, its physicochemical stability and maintenance of sterility over a 6 month period were assessed.
View Article and Find Full Text PDFEscherichia coli transport in two acidic soils: Effect of microbially induced calcite precipitation technology.
J Contam Hydrol
December 2024
Department of Soil Science, Faculty of Agricultural Sciences, University of Guilan, Rasht 41635-1314, Iran.
Microbially induced calcite precipitation (MICP) while neutralizing soil pH, can lead to pore clogging which in turn may reduce bacteria transport. This study aimed to evaluate the effectiveness of the MICP process for E. coli filtration in two acidic soils.
View Article and Find Full Text PDFDistribution characteristics and analysis of fungal aerosol concentration and particle size in air-conditioned wards in Wuhan, China.
Ann Agric Environ Med
December 2024
School of Biomedical Engineering and Imaging, Hubei University of Science and Technology, Hubei, China.
Article Synopsis
- Fungal contamination in hospital air can impact the health of staff, patients, and caregivers, with a study in Wuhan revealing that factors like temperature, humidity, and seasonal changes significantly influence fungal concentration rather than disease type or personnel density.
- The analysis showed that airborne fungal particle sizes are normally distributed, with the highest proportions found in specific size ranges, but the median diameter remained below 3.19 μm across different departments in both winter and summer.
- The findings suggest a need for improved filtration efficiency for specific fungal particle sizes and the use of appropriate antifungal treatments and hygiene practices in hospital air management.
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