We have developed a small-scale method for isolating high-quality chromosomal DNA from Streptomyces species. The entire procedure may be carried out in 2-mL microcentrifuge tubes in one day. It has been tested both quantitatively and qualitatively to ensure reliability and reproducibility. DNA yields from a variety of Streptomyces species ranged from 30 micrograms DNA/50 mg cells to over 225 micrograms DNA/50 mg cells. We used the method to isolate DNA from cells grown in liquid culture, on solid media and from spore suspensions. DNA yields and quality were assessed by spectrophotometry, restriction endonuclease digestion and random-amplified polymorphic DNA-PCR (RAPD-PCR) analysis. These confirmed that this procedure is an efficient method for isolating large amounts of high-quality DNA from a wide range of Streptomyces species.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.2144/98243st05 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Kinetics of the Oxidation of the [2Fe-2S] Cluster in SoxR by Redox-Active Compounds as Studied by Pulse Radiolysis.
Biochemistry
January 2025
The Institute of Scientific and Industrial Research, Osaka University, Mihogaoka 8-1, Osaka, Ibaraki 567-0047, Japan.
SoxR containing a [2Fe-2S] cluster required for its transcription activity functions as a bacterial stress-response sensor that is activated through oxidation by redox-active compounds (RACs). SoxR from (EcSoxR) is activated by nearly all RACs nonspecifically. In contrast, nonenteric SoxRs such as (PaSoxR), and (ScSoxR) activate their target genes in response to RAC including endogenously produced metabolites.
View Article and Find Full Text PDFMechanism of microbial action of the inoculated nitrogen-fixing bacterium for growth promotion and yield enhancement in rice (Oryza sativa L.).
Adv Biotechnol (Singap)
September 2024
College of Agronomy, Hunan Agricultural University, Changsha, 410128, China.
The use of nitrogen-fixing bacteria in agriculture is increasingly recognized as a sustainable method to boost crop yields, reduce chemical fertilizer use, and improve soil health. However, the microbial mechanisms by which inoculation with nitrogen-fixing bacteria enhance rice production remain unclear. In this study, rice seedlings were inoculated with the nitrogen-fixing bacterium R3 (Herbaspirillum) at the rhizosphere during the seedling stage in a pot experiment using paddy soil.
View Article and Find Full Text PDFGlnA3 is able to glutamylate spermine but it is not essential for the detoxification of spermine in .
J Bacteriol
January 2025
Interfaculty Institute of Microbiology and Infection Medicine Tübingen (IMIT), Department of Microbiology and Biotechnology, University of Tübingen, Tübingen, Baden-Württemberg, Germany.
Unlabelled: is well adapted to survive and persist in the infected host, escaping the host's immune response. Since polyamines such as spermine, which are synthesized by infected macrophages, are able to inhibit the growth of , the pathogen needs strategies to cope with these toxic metabolites. The actinomycete , a close relative of makes use of a gamma-glutamylation pathway to functionally neutralize spermine.
View Article and Find Full Text PDFBiocatalytic diversification of abietic acid in Streptomyces.
J Ind Microbiol Biotechnol
January 2025
Department of Chemistry, University of Florida, Gainesville, FL, USA.
Biocatalysis provides access to synthetically challenging molecules and commercially and pharmaceutically relevant natural product analogs while adhering to principles of green chemistry. Cytochromes P450 (P450s) are amongst the most superlative and versatile oxidative enzymes found in nature and are desired regio- and stereoselective biocatalysts, particularly for structurally complex hydrocarbon skeletons. We used 10 genome-sequenced Streptomyces strains, selected based on their preponderance of P450s, to biotransform the bioactive diterpenoid abietic acid.
View Article and Find Full Text PDFBioactivities of secondary metabolites of two actinomycetes Streptomyces parvulus GloL3, and Streptomyces lienomycini SK5, endophytes of two Indian medicinal herbs- Globba marantina L. and Selaginella kraussiana (Kunze) A. Braun.
Braz J Microbiol
January 2025
Microbiology and Microbial Biotechnology Laboratory, Department of Botany and Forestry, Vidyasagar University, 721102, Midnapore, West Bengal, India.
Endophytic actinomycetes are potential sources of novel pharmaceutically active metabolites, significantly advancing natural product research. In the present investigation, secondary metabolites from two endophytic actinomycetes, Streptomyces parvulus GloL3, and Streptomyces lienomycini SK5, isolated from medicinal plant taxa, Globba marantina, and Selaginella kraussiana, exhibited broad-spectrum bioactivity. Ethyl Acetate (EA) extract of SK5 showed antimicrobial activity against nine human pathogens, including Methicillin-resistant Staphylococcus aureus (MRSA), Candida tropicalis, and C.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!