We compared the 14C-ethanol technique and 133Xe-clearance for adipose tissue blood flow measurements in young healthy subjects before and after exercise on an ergometer bicycle. The results showed a decrease in outflow/inflow ratio of 14C-ethanol during the basal situation before the exercise, indicating an increased blood flow. However, there was a great range of values, and no correlation between the 14C-ethanol technique and 133Xe-clearance was found. Our data indicate that the 14C-ethanol technique can not be recommended in its current form.
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http://dx.doi.org/10.1016/s0024-3205(98)00016-2 | DOI Listing |
Lipids Health Dis
November 2011
Organization and Dynamics of Biological Membranes, UMR 5246 ICBMS, CNRS-Université Claude Bernard Lyon 1, Bâtiment Raulin, 43, boulevard du 11 novembre 1918, 69622 Villeurbanne, Cedex, France.
Background: Phospholipase D (PLD) belongs to a lipolytic enzyme subclass which catalyzes the hydrolysis and transesterification of glycerophospholipids at the terminal phosphodiester bond.
Results: In this work, we have studied the substrate specificity of PLDs from germinating sunflower seeds and cultured-soybean cells, using their capacity of transphosphatidylation. In the presence of a nucleophilic acceptor, such as [¹⁴C]ethanol, PLD catalyzes the production of phosphatidyl-[¹⁴C]-ethanol.
BMC Biochem
March 2009
Department of Genetics, Development and Cell Biology, Iowa State University, Ames, IA 50011, USA.
Background: Eukaryotic aldehyde dehydrogenases (ALDHs, EC 1.2.1), which oxidize aldehydes into carboxylic acids, have been classified into more than 20 families.
View Article and Find Full Text PDFFEMS Microbiol Ecol
February 2007
Department of Chemistry & Chemical Biology, Stevens Institute of Technology, NJ 07030, USA.
We developed a procedure to culture microorganisms below freezing point on solid media (cellulose powder or plastic film) with ethanol as the sole carbon source without using artificial antifreezes. Enrichment from soil and permafrost obtained on such frozen solid media contained mainly fungi, and further purification resulted in isolation of basidiomycetous yeasts of the genera Mrakia and Leucosporidium as well as ascomycetous fungi of the genus Geomyces. Contrary to solid frozen media, the enrichment of liquid nutrient solutions at 0 degrees C or supercooled solutions stabilized by glycerol at -1 to -5 degrees C led to the isolation of bacteria representing the genera Polaromonas, Pseudomonas and Arthrobacter.
View Article and Find Full Text PDFJ Physiol
February 2005
Tasmanian Partnership for Advanced Computing, University of Tasmania, Private Bag 37, Hobart 7001, Tasmania, Australia.
Theoretical models for the description of microdialysis outflow:inflow (O/I) ratio for 3H2O and [14C]ethanol were developed, taking into account the nutritive fraction of total blood flow in muscle. The models yielded an approximately exponential decay expression for the O/I ratio, dependent on the physical dimensions of a linear probe (length and radius), the flow rate through the probe, muscle blood flow (including the nutritive fraction) and the diffusion coefficients for the tracer in the probe and muscle. The models compared favourably with experimental data from the constant-flow perfused rat hindlimb.
View Article and Find Full Text PDFLife Sci
January 2002
Department of Surgery, Sahlgrenska University Hospital, Gothenburg, Sweden.
In this study, we estimated interstitial histamine concentrations in normal and malignant tissues after a single intravenous (i.v.) injection of 0.
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