AI Article Synopsis
- The study evaluated ribotyping and ERIC-PCR for differentiating 122 strains of diarrhoeagenic Escherichia coli across 26 serotypes.
- There was a high correlation (about 90%) between the results of ribotyping, ERIC-PCR, and multilocus enzyme electrophoresis for the main ribotypes.
- Similar ribotypes were found in related strains like O55:H7 and O157:H7, indicating that ribotyping and ERIC-PCR can be useful for identifying specific clones of diarrhoeagenic E. coli.
Article Abstract
The ability of ribotyping and enterobacterial repetitive intergenic concensus-polymerase chain reaction (ERIC-PCR) to discriminate diarrhoeagenic Escherichia coli clones of 122 strains belonging to 26 distinct serotypes was evaluated. The 26 serotypes corresponded to 24 ribotypes and 25 ERIC-types. Correlation between multilocus enzyme electrophoresis, ERIC-PCR and ribotyping was c. 90% for the dominant ribotypes. Related clones such as O55:H7 and O157:H7 presented similar ribotypes and clustered together in a dendrogram, and the two divergent clonal groups of enteropathogenic E. coli (EPEC) and enterohaemorrhagic E. coli (EHEC) were included in distinct branches. The results suggest the possibility of applying these two simpler techniques as tools to identify clones of diarrhoeagenic E. coli.
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| http://dx.doi.org/10.1099/00222615-47-3-227 | DOI Listing |
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