[Effect of 135 days human stay in chamber on the composition of electrophoretic fraction and levels of some individual plasma proteins].

The investigation was performed with three test-subjects who were locked in a chamber with simulated spaceflight environment for 135 days. Samples of venous plasma were collected to determine concentrations of total protein, total cholesterol (TCh), cholesterol of high density lipoproteins (HDLP), alpha 1-inhibitor of proteolysis (alpha 1-Pl), alpha 2-microglobulin (alpha 2 M), and haptoglobin (Hp). Concentrations of cholesterol of low density lipoproteins (Ch LDLP) and HDLP-LDLP ratio were calculated. Relative content of electrophoretic fractions of alpha 1- and alpha 2-globulins and a qualitative pattern of electrophoretic separation of lipoproteins were also determined. Data from the investigation showed significant decreases in alpha 1-Pl, alpha 2-M, Hp, and the alpha 2-globulin fraction that could be consequent to suppression of protein synthesis in the liver. Considering that at some points in the investigation concentrations of proteolysis-inhibiting and regulating alpha 1-Pl and alpha 2-M dropped to or went beyond the bottom margin of the norm, these effects have physiological implications. On days 2 and 7 after exit from the chamber, a weak acute-phase reaction was apparently linked with the emotional stress. Two test-subjects had chronologically differing periods of elevated TCh and Ch HDLP. Yet, the HDLP-LDLP ratios have never left boundaries of the physiological norm.